| Texto completo | |
| Autor(es): |
Andrade, Gabriella Mamede
;
da Silveira, Juliano Coelho
;
Perrini, Claudia
;
Del Collado, Maite
;
Gebremedhn, Samuel
;
Tesfaye, Dawit
;
Meirelles, Flavio Vieira
;
Perecin, Felipe
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS One; v. 12, n. 9 SEP 18 2017. |
| Citações Web of Science: | 11 |
| Resumo | |
The ovarian follicle encloses oocytes in a microenvironment throughout their growth and acquisition of competence. Evidence suggests a dynamic interplay among follicular cells and oocytes, since they are constantly exchanging ``messages{''}. We dissected bovine ovarian follicles and recovered follicular cells (FCs-granulosa and cumulus cells) and cumulus-oocyte complexes (COCs) to investigate whether the PI3K-Akt signaling pathway impacted oocyte quality. Following follicle rupture, COCs were individually selected for in vitro cultures to track the follicular cells based on oocyte competence to reach the blastocyst stage after parthenogenetic activation. Levels of PI3K-Akt signaling pathway components in FCs correlated with oocyte competence. This pathway is upregulated in FCs from follicles with high-quality oocytes that are able to reach the blastocyst stage, as indicated by decreased levels of PTEN and increased levels of the PTEN regulators bta-miR-494 and bta-miR-20a. Using PI3K-Akt responsive genes, we showed decreased FOXO3a levels and BAX levels in lower quality groups, indicating changes in cell cycle progression, oxidative response and apoptosis. Based on these results, the measurement of levels of PI3K-Akt pathway components in FCs from ovarian follicles carrying oocytes with distinct developmental competences is a useful tool to identify putative molecular pathways involved in the acquisition of oocyte competence. (AU) | |
| Processo FAPESP: | 12/50533-2 - GIFT: melhoramento genômico de características relacionadas com a fertilização em gado bovino dinamarquês e brasileiro |
| Beneficiário: | Marcelo Fábio Gouveia Nogueira |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 13/08135-2 - CTC - Centro de Terapia Celular |
| Beneficiário: | Dimas Tadeu Covas |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 14/22887-0 - Exossomos e microvesículas contendo miRNAs modulam mudanças epigenéticas durante o cultivo in vitro de gametas e embriões em bovinos |
| Beneficiário: | Juliano Coelho da Silveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 14/21034-3 - Rotas intracelulares e intercelulares durante a foliculogênese e maturação oocitária in vivo e in vitro |
| Beneficiário: | Felipe Perecin |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |