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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes

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Author(s):
Andrade, Gabriella Mamede ; da Silveira, Juliano Coelho ; Perrini, Claudia ; Del Collado, Maite ; Gebremedhn, Samuel ; Tesfaye, Dawit ; Meirelles, Flavio Vieira ; Perecin, Felipe
Total Authors: 8
Document type: Journal article
Source: PLoS One; v. 12, n. 9 SEP 18 2017.
Web of Science Citations: 10
Abstract

The ovarian follicle encloses oocytes in a microenvironment throughout their growth and acquisition of competence. Evidence suggests a dynamic interplay among follicular cells and oocytes, since they are constantly exchanging ``messages{''}. We dissected bovine ovarian follicles and recovered follicular cells (FCs-granulosa and cumulus cells) and cumulus-oocyte complexes (COCs) to investigate whether the PI3K-Akt signaling pathway impacted oocyte quality. Following follicle rupture, COCs were individually selected for in vitro cultures to track the follicular cells based on oocyte competence to reach the blastocyst stage after parthenogenetic activation. Levels of PI3K-Akt signaling pathway components in FCs correlated with oocyte competence. This pathway is upregulated in FCs from follicles with high-quality oocytes that are able to reach the blastocyst stage, as indicated by decreased levels of PTEN and increased levels of the PTEN regulators bta-miR-494 and bta-miR-20a. Using PI3K-Akt responsive genes, we showed decreased FOXO3a levels and BAX levels in lower quality groups, indicating changes in cell cycle progression, oxidative response and apoptosis. Based on these results, the measurement of levels of PI3K-Akt pathway components in FCs from ovarian follicles carrying oocytes with distinct developmental competences is a useful tool to identify putative molecular pathways involved in the acquisition of oocyte competence. (AU)

FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 14/21034-3 - Intra and intercellular routes during foliculogenesis and in vivo and in vitro oocyte maturation
Grantee:Felipe Perecin
Support type: Regular Research Grants
FAPESP's process: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle
Grantee:Marcelo Fábio Gouveia Nogueira
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC