Comparative proteomics reveals recruitment patterns of some protein families in the venoms of Cnidaria

Autor(es)

Jaimes-Becerra, Adrian ; Chung, Ray ; Morandini, Andre C. ; Weston, Andrew J. ; Padilla, Gabriel ; Gacesa, Ranko ; Ward, Malcolm ; Long, Paul F. ; Marques, Antonio C.

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 137, p. 19-26, OCT 2017.
• Citações Web of Science: 5

Resumo

Cnidarians are probably the oldest group of animals to be venomous, yet our current picture of cnidarian venom evolution is highly imbalanced due to limited taxon sampling. High-throughput tandem mass spectrometry was used to determine venom composition of the scyphozoan Chrysaora lactea and two cubozoans Tamoya haplonema and Chiropsalmus quadrumanus. Protein recruitment patterns were then compared against 5 other cnidarian venom proteomes taken from the literature. A total of 28 putative toxin protein families were identified, many for the first time in Cnidaria. Character mapping analysis revealed that 17 toxin protein families with predominantly cytolytic biological activities were likely recruited into the cnidarian venom proteome before the lineage split between Anthozoa and Medusozoa. Thereafter, venoms of Medusozoa and Anthozoa differed during subsequent divergence of cnidarian classes. Recruitment and loss of toxin protein families did not correlate with accepted phylogenetic patterns of Cnidaria. Selective pressures that drive toxin diversification independent of taxonomic positioning have yet to be identified in Cnidaria and now warrant experimental consideration. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/52324-6 - Síntese e lacunas do conhecimento sobre os organismos da zona costeiro-marinha brasileira
• Beneficiário: Antonio Carlos Marques
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 10/50174-7 - Sistemática, ciclo de vida e padrões reprodutivos de medusas (Cnidaria: Medusozoa: Cubozoa e Scyphozoa) na Baixada Santista (São Paulo, Brasil): biodiversidade marinha
• Beneficiário: André Carrara Morandini
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 13/50484-4 - Biodiversity and distribution patterns of the Medusozoa form the South-Western Atlantic
• Beneficiário: Antonio Carlos Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50242-5 - Dimensões da vida marinha: padrões e processos de diversificação em cnidários planctônicos e bentônicos
• Beneficiário: Antonio Carlos Marques
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 10/06927-0 - Biodiversidade e áreas de endemismo de hidroides bentônicos (Cnidaria, Hydrozoa) da costa austral da América do Sul e Antártica
• Beneficiário: Thaís Pires Miranda
• Modalidade de apoio: Bolsas no Brasil - Doutorado