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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Ectopic Expression of Xylella fastidiosa rpfF Conferring Production of Diffusible Signal Factor in Transgenic Tobacco and Citrus Alters Pathogen Behavior and Reduces Disease Severity

Texto completo
Autor(es):
Caserta, R. [1] ; Souza-Neto, R. R. [2, 1] ; Takita, M. A. [1] ; Lindow, S. E. [3] ; De Souza, A. A. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Ctr Citricultura Sylvio Moreira IAC, Corderiopolis, SP - Brazil
[2] Univ Estadual Campinas UNICAMP, Campinas, SP - Brazil
[3] Univ Calif Berkeley, Berkeley, CA - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: MOLECULAR PLANT-MICROBE INTERACTIONS; v. 30, n. 11, p. 866-875, NOV 2017.
Citações Web of Science: 5
Resumo

The pathogenicity of Xylella fastidiosa is associated with its ability to colonize the xylem of host plants. Expression of genes contributing to xylem colonization are suppressed, while those necessary for insect vector acquisition are increased with increasing concentrations of diffusible signal factor (DSF), whose production is dependent on RpfF. We previously demonstrated that transgenic citrus plants ectopically expressing rpfF from a citrus strain of X. fastidiosa subsp. pauca exhibited less susceptibility to Xanthomonas citri subsp. citri, another pathogen whose virulence is modulated by DSF accumulation. Here, we demonstrate that ectopic expression of rpfF in both transgenic tobacco and sweet orange also confers a reduction in disease severity incited by X. fastidiosa and reduces its colonization of those plants. Decreased disease severity in the transgenic plants was generally associated with increased expression of genes conferring adhesiveness to the pathogen and decreased expression of genes necessary for active motility, accounting for the reduced population sizes achieved in the plants, apparently by limiting pathogen dispersal through the plant. Plant-derived DSF signal molecules in a host plant can, therefore, be exploited to interfere with more than one pathogen whose virulence is controlled by DSF signaling. (AU)

Processo FAPESP: 13/10957-0 - Interação Xylella fastidiosa-inseto vetor-planta hospedeira e abordagens para o controle da clorose variegada dos citros e cancro cítrico
Beneficiário:Alessandra Alves de Souza
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 08/57909-2 - Plataforma genômica aplicada ao melhoramento de citros
Beneficiário:Marcos Antonio Machado
Linha de fomento: Auxílio à Pesquisa - Temático