Erythrosine B and quinoline yellow dyes regulate DNA repair gene expression in human HepG2 cells

Autor(es)

Chequer, Farah M. D. ^{[1, 2]} ; Venancio, Vinicius P. ^{[1, 3]} ; Almeida, Mara R. ^[1] ; Aissa, Alexandre F. ^[1] ; Bianchi, Maria Lourdes P. ^[1] ; Antunes, Lusania M. G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil

^[2] Univ Fed Minas Gerais, Dept Anal Clin & Toxicol, Belo Horizonte, MG - Brazil

^[3] Texas A&M Univ, Dept Nutr & Food Sci, College Stn, TX 77843 - USA

• Tipo de documento: Artigo Científico
• Fonte: TOXICOLOGY AND INDUSTRIAL HEALTH; v. 33, n. 10, p. 765-774, OCT 2017.
• Citações Web of Science: 0

Resumo

Erythrosine B (ErB) is a cherry pink food colorant and is widely used in foods, drugs, and cosmetics. Quinoline yellow (QY) is a chinophthalon derivative used in cosmetic compositions for application to the skin, lips, and/or body surface. Previously, ErB and QY synthetic dyes were found to induce DNA damage in HepG2 cells. The aim of this study was to investigate the molecular basis underlying the genotoxicity attributed to ErB and QY using the RT2 Profiler polymerase chain reaction array and by analyzing the expression profile of 84 genes involved in cell cycle arrest, apoptosis, and DNA repair in HepG2 cells. ErB (70 mg/L) significantly decreased the expression of two genes (FEN1 and REV1) related to DNA base repair. One gene (LIG1) was downregulated and 20 genes related to ATR/ATM signaling (ATR, RBBP8, RAD1, CHEK1, CHEK2, TOPB1), nucleotide excision repair (ERCC1, XPA), base excision repair (FEN1, MBD4), mismatch repair (MLH1, MSH3, TP73), double strand break repair (BLM), other DNA repair genes (BRIP1, FANCA, GADD45A, REV1), and apoptosis (BAX, PPP1R15A) were significantly increased after treatment with QY (20 mg/L). In conclusion, our data suggest that the genotoxic mechanism of ErB and QY dyes involves the modulation of genes related to the DNA repair system and cell cycle. (AU)

• Processo FAPESP: 11/14115-9 - Avaliação toxicogenética, análise eletroquímica e perfil da expressão de genes associados aos danos no DNA em células HepG2 expostas aos corantes para alimentos eritrosina, quinolina amarela e seus subprodutos
• Beneficiário: Lusânia Maria Greggi Antunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/01755-0 - Avaliação toxicogenética dos corantes de alimentos eritrosina e quinolina amarela e seus subprodutos: análise eletroquímica e da expressão dos genes TP53 e COX-2 em células hepáticas
• Beneficiário: Farah Maria Drumond Chequer
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado