Ion Mobility-Enhanced Data-Independent Acquisitions Enable a Deep Proteomic Landscape of Oligodendrocytes

Autor(es)

Cassoli, Juliana S. ^[1] ; Brandao-Teles, Caroline ^[1] ; Santana, Aline G. ^[1] ; Souza, Gustavo H. M. F. ^[2] ; Martins-de-Souza, Daniel ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Lab Neuroprote, Campinas, SP - Brazil

^[2] Waters Corp, Hlth Sci Dept, Mass Spectrometry Res & Dev Lab, Sao Paulo, SP - Brazil

^[3] Conselho Nacl Desenvolvimento Cient & Tecnol, Inst Nacl Biomarcadores Neuropsiquiatria INBION, Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PROTEOMICS; v. 17, n. 21 NOV 2017.
• Citações Web of Science: 4

Resumo

Oligodendrocytes are a type of neuroglia that provide trophic support and insulation to axons in the central nervous system. The genesis and maturation of oligodendrocytes are essential processes for myelination and the course of CNS development. Using ion mobility-enhanced, data-independent acquisitions and 2D-nanoUPLC fractionation operating at nanoscale flow rates, we established a comprehensive data set of proteins expressed by the human oligodendroglia cell line MO3.13. The final dataset incorporating all fractions comprised 223 531 identified peptides assigned to 10 390 protein hits, an improvement of 4.5 times on identified proteins described previously by our group using the same cell line. Identified proteins play pivotal roles in many biological processes such as cell growth and development and energy metabolism, providing a rich resource for future studies on oligodendrocyte development, myelination, axonal support, and the regulation of such process. Our results can help further studies that use MO3.13 cells as a tool of investigation, not only in relation to oligodendrocyte maturation, but also to diseases that have oligodendrocytes as key players. All MS data have been deposited in the ProteomeXchange with identifier PXD004696. (AU)

• Processo FAPESP: 14/14881-1 - Compreensão da influência de componentes da via glicolítica na função dos oligodendrócitos: uma relação com os achados em esquizofrenia
• Beneficiário: Juliana Silva Cassoli
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/23049-0 - Unindo culturas de oligodendrócitos humanos e disfunção do sistema glutamatérgico para compreender as bases moleculares da esquizofrenia
• Beneficiário: Caroline Brandão Teles Rodrigues
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/08711-3 - Desenvolvimento de um teste preditivo para medicação bem sucedida e compreensão das bases moleculares da esquizofrenia através da proteômica
• Beneficiário: Daniel Martins-de-Souza
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 14/10068-4 - EMU concedido no processo 13/08711-3: espectrômetro de massas Waters SYNAPT G2-Si HDMs + nanoACQUITY UPLC
• Beneficiário: Daniel Martins-de-Souza
• Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários