Is DNA ploidy related to smoking?

Autor(es)

Lima, Celina Faig ^{[1, 2]} ; Oliveira Alves, Monica Ghislaine ^{[1, 2]} ; do Carmo Carvalho, Bruna Fernandes ^[1] ; de Lima, Thaynara Alves ^[1] ; Coutinho-Camillo, Claudia Malheiros ^[3] ; Soares, Fernando Augusto ^[3] ; Scholz, Jaqueline ^[4] ; Almeida, Janete Dias ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil

^[2] Univ Braz Cubas, Sch Dent, Mogi Das Cruzes - Brazil

^[3] AC Camargo Canc Ctr, Int Res Ctr, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Univ Hosp, Heart Inst Sao Paulo, Med Sch, Outpatient Program Treatment Smoking, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL PATHOLOGY & MEDICINE; v. 46, n. 10, p. 961-966, NOV 2017.
• Citações Web of Science: 0

Resumo

BackgroundIn the oral cavity, genomic instability is caused by long-term exposure to carcinogens. The aim of this study was to evaluate the relationship between smoking and DNA ploidy. MethodsCytological material was obtained from patients participating in the Outpatient Smoking Treatment Program of the Heart Institute (INCOR-HCFMUSP), and of the Discipline of Oral Medicine (ICT-UNESP). The inclusion criteria for all groups were the absence of a history of malignant tumors, absence of clinical signs of changes in the selected area, and alcohol consumption of less than 3 units per week. Group 1:30 smokers before smoking cessation treatment; Group 2:30 non-smokers; Group 3:30 ex-smokers abstinent for at least 1 year. Cytological smears were collected from the floor of the mouth and border of the tongue and stained by Feulgen. Aneuploidy was evaluated using the ACIS (R) III system. ResultsThe Kruskal-Wallis test showed no statistically significant difference (P = .4383) between the groups studied. No association between tobacco consumption and aneuploidy was observed in group 1 (P = 1) or group 2 (P = .68; Fisher's exact test). ConclusionSmoking was not associated with changes in DNA content or the incidence of aneuploidy in normal oral mucosa. (AU)

• Processo FAPESP: 12/05371-4 - Avaliação citogenética dos danos causados pelo tabagismo à mucosa bucal
• Beneficiário: Janete Dias Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/09910-0 - Avaliação do efeito do tabagismo crônico e da abstinência na aneuploidia em células da mucosa bucal
• Beneficiário: Bruna Fernandes do Carmo Carvalho
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/02168-6 - Efeito do tabagismo crônico na metilação dos genes de reparo de dna da mucosa bucal.
• Beneficiário: Celina Faig Lima Carta
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado