miR18a and miR19a Recruit Specific Proteins for Splicing in Thyroid Cancer Cells

Autor(es)

Paiva, Marcelo M. ^[1] ; Kimura, Edna T. ^[1] ; Coltri, Patricia P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CANCER GENOMICS & PROTEOMICS; v. 14, n. 5, p. 373-381, SEP-OCT 2017.
• Citações Web of Science: 3

Resumo

Background: Thyroid cancer is one of the most frequent types of endocrine cancers. In most cases, thyroid cancers are caused by deregulated miRNA expression, especially involving the miR17-92 cluster. miR17-92 transcription is altered in several different tumor types including lymphoma, leukemia, and of the breast and thyroid. As an intronic cluster, miR17-92 must be processed during splicing and therefore interaction between microprocessor and spliceosome machineries is of major importance in understanding its expression. Materials and Methods: We investigated the protein composition of spliceosomes assembled on pre-RNAs containing intronic miR18a and miR19a, components of the miR17-92 cluster, using mass spectrometry. Results: Interestingly, we observed that proteins associated with intronic miR18a and miR19a are cell-specific, and are similar for both miRNAs analyzed. The only exception is the group of heterogeneous nuclear proteins that are commonly recruited by different cells. Conclusion: miRNA processing depends on cell-specific proteins and heterogeneous nuclear proteins have a general role in miRNA processing from introns. (AU)

• Processo FAPESP: 17/06994-9 - Regulação do splicing de microRNAs em eucariotos
• Beneficiário: Patricia Pereira Coltri
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/02738-7 - Caracterização do papel de proteínas reguladoras do splicing em eucariotos
• Beneficiário: Patricia Pereira Coltri
• Modalidade de apoio: Auxílio à Pesquisa - Regular