| Full text | |
| Author(s): |
Total Authors: 3
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| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 1
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| Document type: | Journal article |
| Source: | CANCER GENOMICS & PROTEOMICS; v. 14, n. 5, p. 373-381, SEP-OCT 2017. |
| Web of Science Citations: | 3 |
| Abstract | |
Background: Thyroid cancer is one of the most frequent types of endocrine cancers. In most cases, thyroid cancers are caused by deregulated miRNA expression, especially involving the miR17-92 cluster. miR17-92 transcription is altered in several different tumor types including lymphoma, leukemia, and of the breast and thyroid. As an intronic cluster, miR17-92 must be processed during splicing and therefore interaction between microprocessor and spliceosome machineries is of major importance in understanding its expression. Materials and Methods: We investigated the protein composition of spliceosomes assembled on pre-RNAs containing intronic miR18a and miR19a, components of the miR17-92 cluster, using mass spectrometry. Results: Interestingly, we observed that proteins associated with intronic miR18a and miR19a are cell-specific, and are similar for both miRNAs analyzed. The only exception is the group of heterogeneous nuclear proteins that are commonly recruited by different cells. Conclusion: miRNA processing depends on cell-specific proteins and heterogeneous nuclear proteins have a general role in miRNA processing from introns. (AU) | |
| FAPESP's process: | 17/06994-9 - MicroRNA splicing regulation in eukaryotes |
| Grantee: | Patricia Pereira Coltri |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/02738-7 - Characterization of the role of proteins involved in splicing regulation in eukaryotes |
| Grantee: | Patricia Pereira Coltri |
| Support Opportunities: | Regular Research Grants |