Preparation, characterization and in vitro evaluation of epsilon-polylysine-loaded polymer blend microparticles for potential pancreatic cancer therapy

Autor(es)

Chevalier, Merari T. ^[1] ; Garcia, Monica C. ^[2] ; Gonzalez, Daniela ^[3] ; Gomes-Filho, Sandro M. ^[4] ; Basseres, Daniela S. ^[4] ; Farina, Hernan ^[5] ; Alvareza, Vera A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Nacl Mar Del Plata, Inst Invest Cienc & Tecnol Matriales INTEMA, Grp Mat Compuestos Matriz Termoplast, Colon 10890, RA-7600 Mar Del Plata, Buenos Aires - Argentina

^[2] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Farmacia, Unidad Invest & Dessarollo Tecnol Farmaceut UNITE, Ciudad Univ, Cordoba - Argentina

^[3] Univ Nebraska Lincoln, Dept Chem & Biomol Engn, Lincoln, NE - USA

^[4] Univ Sao Paulo, Chem Inst, Dept Biochem, Sao Paulo, SP - Brazil

^[5] Natl Univ Quilmes, Lab Mol Oncol, Quilmes - Argentina

• Tipo de documento: Artigo Científico
• Fonte: Journal of Microencapsulation; v. 34, n. 6, p. 582-591, 2017.
• Citações Web of Science: 2

Resumo

Peptide active ingredients show great promise regarding the treatment of various health-endangering diseases. It is reported that L-lysine inhibits the proliferation of several tumour lines in vitro and in vivo. However, proteins and peptide drugs possess certain disadvantages such as in vivo instability and short biological half-life. On the grounds that drug delivery systems can overcome a wide spectrum of bioactive compounds issues, a biopolymeric blend-based micro-particulated system capable of delivering epsilon-polylysine (PLL) was developed. PLL-loaded poly((L)Lactic acid)/poly(D,L-Lactide)-co-poly(ethylene glycol)-based microparticles (PLL-PB-MPs) were prepared and fully characterised exhibiting a narrow size distribution (1.2 +/- 0.12 mu m), high loading efficiency (81%) and improved thermal stability (T-d from 250 degrees C to 291 degrees C). The cytotoxicity and antiproliferative effect of PLL-PB-MPs in pancreatic adenocarcinoma cell lines BxPC3 and MIA PaCa-2 were confirmed. Due to their physicochemical and biopharmaceutical properties, PB-MPs constitute a promising carrier to deliver bioactive peptides. (AU)

• Processo FAPESP: 10/52685-9 - Novos alvos terapêuticos no câncer de pulmão associado a mutações no oncogênese K-Ras
• Beneficiário: Daniela Sanchez Basseres
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores