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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Preparation, characterization and in vitro evaluation of epsilon-polylysine-loaded polymer blend microparticles for potential pancreatic cancer therapy

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Author(s):
Chevalier, Merari T. [1] ; Garcia, Monica C. [2] ; Gonzalez, Daniela [3] ; Gomes-Filho, Sandro M. [4] ; Basseres, Daniela S. [4] ; Farina, Hernan [5] ; Alvareza, Vera A. [1]
Total Authors: 7
Affiliation:
[1] Univ Nacl Mar Del Plata, Inst Invest Cienc & Tecnol Matriales INTEMA, Grp Mat Compuestos Matriz Termoplast, Colon 10890, RA-7600 Mar Del Plata, Buenos Aires - Argentina
[2] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Farmacia, Unidad Invest & Dessarollo Tecnol Farmaceut UNITE, Ciudad Univ, Cordoba - Argentina
[3] Univ Nebraska Lincoln, Dept Chem & Biomol Engn, Lincoln, NE - USA
[4] Univ Sao Paulo, Chem Inst, Dept Biochem, Sao Paulo, SP - Brazil
[5] Natl Univ Quilmes, Lab Mol Oncol, Quilmes - Argentina
Total Affiliations: 5
Document type: Journal article
Source: Journal of Microencapsulation; v. 34, n. 6, p. 582-591, 2017.
Web of Science Citations: 2
Abstract

Peptide active ingredients show great promise regarding the treatment of various health-endangering diseases. It is reported that L-lysine inhibits the proliferation of several tumour lines in vitro and in vivo. However, proteins and peptide drugs possess certain disadvantages such as in vivo instability and short biological half-life. On the grounds that drug delivery systems can overcome a wide spectrum of bioactive compounds issues, a biopolymeric blend-based micro-particulated system capable of delivering epsilon-polylysine (PLL) was developed. PLL-loaded poly((L)Lactic acid)/poly(D,L-Lactide)-co-poly(ethylene glycol)-based microparticles (PLL-PB-MPs) were prepared and fully characterised exhibiting a narrow size distribution (1.2 +/- 0.12 mu m), high loading efficiency (81%) and improved thermal stability (T-d from 250 degrees C to 291 degrees C). The cytotoxicity and antiproliferative effect of PLL-PB-MPs in pancreatic adenocarcinoma cell lines BxPC3 and MIA PaCa-2 were confirmed. Due to their physicochemical and biopharmaceutical properties, PB-MPs constitute a promising carrier to deliver bioactive peptides. (AU)