Alternagin-C (ALT-C), a Disintegrin-Like Cys-Rich Protein Isolated from the Venom of the Snake Rhinocerophis alternatus, Stimulates Angiogenesis and Antioxidant Defenses in the Liver of Freshwater Fish, Hoplias malabaricus

Autor(es)

Monteiro, Diana Amaral ^[1] ; Selistre-de-Araujo, Heloisa Sobreiro ^[1] ; Tavares, Driele ^[1] ; Fernandes, Marisa Narciso ^[1] ; Kalinin, Ana Lucia ^[1] ; Rantin, Francisco Tadeu ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Carlos UFSCar, Dept Physiol Sci, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXINS; v. 9, n. 10 OCT 2017.
• Citações Web of Science: 2

Resumo

Alternagin-C (ALT-C) is a disintegrin-like protein isolated from Rhinocerophis alternatus snake venom, which induces endothelial cell proliferation and angiogenesis. The aim of this study was to evaluate the systemic effects of a single dose of alternagin-C (0.5 mg<bold>kg</bold>-1, via intra-arterial) on oxidative stress biomarkers, histological alterations, vascular endothelial growth factor (VEGF) production, and the degree of vascularization in the liver of the freshwater fish traira, Hoplias malabaricus, seven days after the initiation of therapy. ALT-C treatment increased VEGF levels and hepatic angiogenesis. ALT-C also enhanced hepatic antioxidant enzymes activities such as superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, decreasing the basal oxidative damage to lipids and proteins in the fish liver. These results indicate that ALT-C improved hepatic tissue and may play a crucial role in tissue regeneration mechanisms. (AU)

• Processo FAPESP: 13/00798-2 - A matriz extracelular no envelhecimento, no exercício e no microambiente tumoral
• Beneficiário: Heloisa Sobreiro Selistre de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/10993-4 - Efeitos da alternagina-C (ALT-C), uma proteína extraída do veneno da serpente Urutu (Rhinocerophis alternatus, antiga Bothrops alternatus), sobre a contratilidade cardíaca de peixe em normóxia e frente à hipóxia tecidual
• Beneficiário: Diana Amaral Monteiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado