Effects of soy isoflavones on the concentration of hyaluronic acid in the vagina of type 1 diabetic rats

Autor(es)

Carbonel, A. A. F. ^[1] ; Azevedo Lima, P. D. ^[2] ; Lim, J. J. ^[2] ; Teixeira Borges, F. ^[3] ; Rodrigues da Silva Sasso, G. ^[1] ; Portugal Fuchs, L. F. ^[4] ; Simoes, R. S. ^[4] ; Chada Baracat, E. ^[4] ; Soares, Jr., J. M. ^[4] ; Simoes, M. J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, EPM UNIFESP, Dept Morphol & Genet, Paulista Sch Med, Sao Paulo - Brazil

^[2] Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON - Canada

^[3] Univ Fed Sao Paulo, EPM UNIFESP, Dept Nephrol, Paulista Sch Med, Sao Paulo - Brazil

^[4] Univ Sao Paulo, FMUSP, Dept Obstet & Gynecol, Med Fac, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLIMACTERIC; v. 20, n. 6, p. 564-570, 2017.
• Citações Web of Science: 1

Resumo

Objective: To assess the effects of isoflavones and 17-estradiol on the vaginal epithelium extracellular matrix and hyaluronic acid (HA) in the diabetic rat model.Methods: Sixty adult, virgin, female rats underwent ovariectomy, then randomization into six groups of ten animals each: GI, sham ovariectomized control animals; GII, sham ovariectomized control diabetic animals; GIII, control ovariectomized rats receiving propylene glycol vehicle; GIV, control ovariectomized diabetic animals receiving propylene glycol vehicle; GV, diabetic ovariectomized animals treated with soy isoflavones (150mg/kg by gavage); GVI, ovariectomized diabetic rats treated with estrogen (17-estradiol, 10mg/kg, subcutaneously). Treatment took place over 30 consecutive days. After euthanasia, a portion of the vagina was immersed in liquid nitrogen for RT-qPCR and Western blotting. Another portion was processed for paraffin embedding. Sections were stained with hematoxylin \& eosin for histomorphometry and Picro Sirius Red for collagen quantification.Results: Vaginal epithelium histomorphometry in GIII (15.31.1 mu m) and GIV (14.5 +/- 1.8 mu m) was thinner than in GV (41.3 +/- 1.5 mu m) and GVI (74.3 +/- 1.6 mu m). There was an increase in collagen content in GV (84.1 +/- 1.2 mu m) and GVI (88.2 +/- 1.7 mu m). HA quantification was higher in GV (0.38 +/- 1.1g/mg) and GVI (0.49 +/- 1.4g/mg) when compared with GIII (0.12 +/- 1.1g/mg) and GIV (0.10 +/- 1.2g/mg), p<0.05. Conclusions: Soy isoflavones increase hyaluronic acid concentration in the vagina of diabetic ovariectomized rats. Such findings might help to attenuate the effects of vulvovaginal atrophy in women. (AU)

• Processo FAPESP: 14/17077-9 - Efeitos das isoflavonas ou 17beta-estradiol na interação matriz-célula no endométrio de ratas diabéticas: relevância para a terapia hormonal
• Beneficiário: Adriana Aparecida Carbonel Castro Ferreira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado