| Full text | |
| Author(s): |
Carbonel, A. A. F.
[1]
;
Azevedo Lima, P. D.
[2]
;
Lim, J. J.
[2]
;
Teixeira Borges, F.
[3]
;
Rodrigues da Silva Sasso, G.
[1]
;
Portugal Fuchs, L. F.
[4]
;
Simoes, R. S.
[4]
;
Chada Baracat, E.
[4]
;
Soares, Jr., J. M.
[4]
;
Simoes, M. J.
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Fed Sao Paulo, EPM UNIFESP, Dept Morphol & Genet, Paulista Sch Med, Sao Paulo - Brazil
[2] Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON - Canada
[3] Univ Fed Sao Paulo, EPM UNIFESP, Dept Nephrol, Paulista Sch Med, Sao Paulo - Brazil
[4] Univ Sao Paulo, FMUSP, Dept Obstet & Gynecol, Med Fac, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | CLIMACTERIC; v. 20, n. 6, p. 564-570, 2017. |
| Web of Science Citations: | 1 |
| Abstract | |
Objective: To assess the effects of isoflavones and 17-estradiol on the vaginal epithelium extracellular matrix and hyaluronic acid (HA) in the diabetic rat model.Methods: Sixty adult, virgin, female rats underwent ovariectomy, then randomization into six groups of ten animals each: GI, sham ovariectomized control animals; GII, sham ovariectomized control diabetic animals; GIII, control ovariectomized rats receiving propylene glycol vehicle; GIV, control ovariectomized diabetic animals receiving propylene glycol vehicle; GV, diabetic ovariectomized animals treated with soy isoflavones (150mg/kg by gavage); GVI, ovariectomized diabetic rats treated with estrogen (17-estradiol, 10mg/kg, subcutaneously). Treatment took place over 30 consecutive days. After euthanasia, a portion of the vagina was immersed in liquid nitrogen for RT-qPCR and Western blotting. Another portion was processed for paraffin embedding. Sections were stained with hematoxylin \& eosin for histomorphometry and Picro Sirius Red for collagen quantification.Results: Vaginal epithelium histomorphometry in GIII (15.31.1 mu m) and GIV (14.5 +/- 1.8 mu m) was thinner than in GV (41.3 +/- 1.5 mu m) and GVI (74.3 +/- 1.6 mu m). There was an increase in collagen content in GV (84.1 +/- 1.2 mu m) and GVI (88.2 +/- 1.7 mu m). HA quantification was higher in GV (0.38 +/- 1.1g/mg) and GVI (0.49 +/- 1.4g/mg) when compared with GIII (0.12 +/- 1.1g/mg) and GIV (0.10 +/- 1.2g/mg), p<0.05. Conclusions: Soy isoflavones increase hyaluronic acid concentration in the vagina of diabetic ovariectomized rats. Such findings might help to attenuate the effects of vulvovaginal atrophy in women. (AU) | |
| FAPESP's process: | 14/17077-9 - Effects of isoflavones or 17beta-estradiol on cell-matrix interactions in the endometrium of diabetic rats: relevance for hormone therapy |
| Grantee: | Adriana Aparecida Carbonel Castro Ferreira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |