First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (acai) in cultured human cells

Autor(es)

Marques, E. S. ^[1] ; Tsuboy, M. S. F. ^[2] ; Carvalho, J. C. T. ^[3] ; Rosa, P. C. P. ^[4] ; Perazzo, F. F. ^[5] ; Gaivao, I. O. M. ^{[6, 7]} ; Maistro, E. L. ^{[1, 8]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Biociencias, Programa Posgrad Biol Geral & Aplicada, Botucatu, SP - Brazil

^[2] Univ Estadual Paulista, Fac Ciencias & Letras, Dept Biotecnol, Assis, SP - Brazil

^[3] Univ Fed Amapa, Colegiado Farm, Ctr Ciencias Biol & Saude, Lab Pesquisa Farmacos, Macapa, AP - Brazil

^[4] Univ Estadual Campinas, Fac Ciencias Med, Campinas, SP - Brazil

^[5] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Exatas & Terra, Diadema, SP - Brazil

^[6] Tras Os Montes & Alto Douro Univ, CECAV, Vila Real - Portugal

^[7] Tras Os Montes & Alto Douro Univ, Dept Genet & Biotechnol, Vila Real - Portugal

^[8] Univ Estadual Paulista, Fac Filosofia & Ciencia, Dept Fonoaudiol, Marilia, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 16, n. 3 AUG 18 2017.
• Citações Web of Science: 3

Resumo

Euterpe oleracea Mart., popularly known as ``acai{''}, is a tropical fruit from the Amazon region where it has considerable economic importance. Acai has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and antigenotoxic effects of E. oleracea fruit oil (EOO) in cultured human lymphocytes (non-metabolizing cells) and HepG2 cell line (human hepatoma) (metabolizing cells) by using MTT, comet, and micronucleus assays. A wide range of EOO concentrations was tested with a preliminary MTT assay, which allowed selecting five concentrations for comet and micronucleus assays: 2.5, 10, 100, 500, and 1000 mu g/mL. The results showed that none of the EOO tested concentrations presented cytotoxic effects. The genotoxic assessment revealed an absence of significant DNA and chromosome damage in human lymphocytes and HepG2 cells but did not show chemoprotection against the DNA damage induced by methyl methanesulfonate and benzo{[}a] pyrene, used as DNA-damaging agents. (AU)

• Processo FAPESP: 12/17241-8 - Investigação dos efeitos citotóxico, genotóxico, antigenotóxico e antioxidante do óleo de Euterpe oleracea Mart. (açaí): análises in vitro e in vivo, histológicas, ultra-estruturais e hematológicas
• Beneficiário: Edson Luis Maistro
• Modalidade de apoio: Auxílio à Pesquisa - Regular