Proteomic approaches for drug discovery against tegumentary leishmaniasis

Autor(es)

Negrao, Fernanda ^{[1, 2]} ; Eberlin, Marcos Nogueira ^[1] ; Giorgio, Selma ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Anim Biol, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: BIOMEDICINE & PHARMACOTHERAPY; v. 95, p. 577-582, NOV 2017.
• Citações Web of Science: 4

Resumo

Tegumentary leishmaniases (TL) comprise various clinical forms, in which current therapeutic treatments lack in safety and efficacy. Recently the parasite is developing resistance mechanisms against anti leishmanial drugs startling the scientific community to recruit efforts to search for novel therapeutics. Proteomics hold promises for the treatment of leishmaniasis and investigation of parasite-host interaction since these set of methodological tools have provided a wealth of protein expression data on several Leishmania species. Firstly this review puts together the current treatment and challenges to fight tegumentary leishmaniasis. In addition, the 2 dimensional gel electrophoresis and mass spectrometry techniques in protein identification and characterization are described and discussed in the context of proteomics regarding Leishmania studies. In this review, we selected literature content on TL causative agents. Important proteomic findings related to differentiation proteome (promastigote and amastigote forms), Leishmania-macrophage interaction proteome and secreted and soluble proteins including molecules involved in parasite resistance and potential drug targets are examined and discussed. We also highlight open questions regarding drug research that can be addressed with proteomics approaches. (AU)

• Processo FAPESP: 16/11517-2 - Busca e identificação de candidatos a biomarcadores da leishmaniose cutânea através de espectrometria de massas
• Beneficiário: Fernanda Negrão Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 15/23767-0 - Pesquisa de biomarcadores e desenvolvimento de linhagens de Leishmania infantum
• Beneficiário: Selma Giorgio
• Modalidade de apoio: Auxílio à Pesquisa - Regular