Lp25 membrane protein from pathogenic Leptospira spp. is associated with rhabdomyolysis and oliguric acute kidney injury in a guinea pig model of leptospirosis

Autor(es):Mostrar menos -

Abreu, Patricia A. E. ^[1] ; Seguro, Antonio C. ^[2] ; Canale, Daniele ^[2] ; da Silva, Ana Maria G. ^[3] ; Matos, Larissa do R. B. ^[1] ; Gotti, Tatiane B. ^[1] ; Monaris, Denize ^[1] ; de Jesus, Denise A. ^[2] ; Vasconcellos, Silvio A. ^[4] ; de Brito, Thales ^[3] ; Magaldi, Antonio J. B. ^[2]

Afiliação do(s) autor(es)

^[1] Butantan Inst, Lab Bacteriol, Sao Paulo - Brazil

^[2] Clin Hosp, Lab Med Invest LIM12, Nephrol Dept, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Trop Med, Med Sch, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Vet Med & Anim Sci, Lab Bacterial Zoonosis, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS Neglected Tropical Diseases; v. 11, n. 5 MAY 2017.
• Citações Web of Science: 5

Resumo

Acute kidney injury (AKI) from leptospirosis is frequently nonoliguric with hypo- or normokalemia. Higher serum potassium levels are observed in non-survivor patients and may have been caused by more severe AKI, metabolic disarrangement, or rhabdomyolysis. An association between the creatine phosphokinase (CPK) level and maximum serum creatinine level has been observed in these patients, which suggests that rhabdomyolysis contributes to severe AKI and hyperkalemia. LipL32 and Lp25 are conserved proteins in pathogenic strains of Leptospira spp., but these proteins have no known function. This study evaluated the effect of these proteins on renal function in guinea pigs. Lp25 is an outer membrane protein that appears responsible for the development of oliguric AKI associated with hyperkalemia induced by rhabdomyolysis (e.g., elevated CPK, uric acid and serum phosphate). This study is the first characterization of a leptospiral outer membrane protein that is associated with severe manifestations of leptospirosis. Therapeutic methods to attenuate this protein and inhibit rhabdomyolysis-induced AKI could protect animals and patients from severe forms of this disease and decrease mortality. (AU)

• Processo FAPESP: 10/20525-2 - Avaliação do potencial imunogênico e vacinal das flagelinas de Leptospira interrogans sorovar Copenhageni.
• Beneficiário: Denize Monaris
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/17695-6 - Desenvolvimento de um teste dipstick para o diagnóstico da leptospirose animal
• Beneficiário: Tatiana Barrionuevo Gotti
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/54701-6 - Desenvolvimento de um vetor para expressão de lipoproteínas de Leptospira interrogans em Escherichia coli
• Beneficiário: Patricia Antonia Estima Abreu de Aniz
• Modalidade de apoio: Auxílio à Pesquisa - Regular