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Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice

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Bagarolli, Renata A. [1] ; Tobar, Natalia [1] ; Oliveira, Alexandre G. [2] ; Araujo, Tiago G. [1] ; Carvalho, Bruno M. [3] ; Rocha, Guilherme Z. [1] ; Vecina, Juliana F. [1] ; Calisto, Kelly [1] ; Guadagnini, Dioze [1] ; Prada, Patricia O. [1] ; Santos, Andrey [1] ; Saad, Sara T. O. [1] ; Saad, Mario J. A. [1]
Número total de Autores: 13
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Dept Internal Med, BR-13081970 Campinas, SP - Brazil
[2] Sao Paulo State Univ UNESP, Biosci Inst, Dept Phys Educ, Rio Claro, SP - Brazil
[3] Univ Fed Pernambuco, Dept Biol Sci, Recife, PE - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Citações Web of Science: 32

Obesity and type 2 diabetes are characterized by subclinical inflammatory process. Changes in composition or modulation of the gut microbiota may play an important role in the obesity-associated inflammatory process. In the current study, we evaluated the effects of probiotics (Lactobacillus rhamnosus, L. acidophilus and Bifidobacterium bifidumi) on gut microbiota, changes in permeability, and insulin sensitivity and signaling in high-fat diet and control animals. More importantly, we investigated the effects of these gut modulations on hypothalamic control of food intake, and insulin and leptin signaling. Swiss mice were submitted to a high-fat diet (HFD) with probiotics or pair-feeding for 5 weeks. Metagenome analyses were performed on DNA samples from mouse feces. Blood was drawn to determine levels of glucose, insulin, LPS, cytokines and GLP-1. Liver, muscle, ileum and hypothalamus tissue proteins were analyzed by Western blotting and real-time polymerase chain reaction. In addition, liver and adipose tissues were analyzed using histology and immunohistochemistry. The HFD induced huge alterations in gut microbiota accompanied by increased intestinal permeability, LPS translocation and systemic low-grade inflammation, resulting in decreased glucose tolerance and hyperphagic behavior. All these obesity-related features were reversed by changes in the gut microbiota profile induced by probiotics. Probiotics also induced an improvement in hypothalamic insulin and leptin resistance. Our data demonstrate that the intestinal microbiome is a key modulator of inflammatory and metabolic pathways in both peripheral and central tissues. These findings shed light on probiotics as an important tool to prevent and treat patients with obesity and insulin resistance. (C) 2017 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 16/07122-2 - Obesidade e resistência à insulina: efeito da iNOS sobre a microbiota intestinal e estresse de retículo endoplasmático em fígado e tecido adiposo de camundongos
Beneficiário:Tamires Marques Zanotto
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 12/15009-0 - O envolvimento da óxido nítrico sintase induzível (iNOS) sobre o estresse de retículo endoplasmático na obesidade induzida por dieta hiperlipídica
Beneficiário:Tamires Marques Zanotto
Linha de fomento: Bolsas no Brasil - Iniciação Científica