Decreased MicroRNA miR-181c Expression Associated with Gastric Cancer

Autor(es)

Zabaglia, Luanna Munhoz ^[1] ; Bartolomeu, Nicole Chiuso ^[1] ; dos Santos, Monica Pezenatto ^[1] ; Peruquetti, Rita Luiza ^[1] ; Chen, Elizabeth ^[2] ; Cardoso Smith, Marilia de Arruda ^[2] ; Marques Payao, Spencer Luiz ^[1] ; Rasmussen, Lucas Trevizani ^[1]

Afiliação do(s) autor(es)

^[1] USC, Proreitoria Pesquisa & Posgrad, Irma Arminda 10-50, BR-17011160 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo UNIFESP, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF GASTROINTESTINAL CANCER; v. 49, n. 1, p. 97-101, MAR 2018.
• Citações Web of Science: 9

Resumo

This study investigated miRNA-181c expression in control patients (healthy gastric mucosa), patients with gastritis, and patients with gastric cancer. The presence of Helicobacter pylori was determined, and the associations between H. pylori infection, levels of miRNA-181c expression, and gastric disease were also analyzed. A total of 158 subjects were included in the study, and the three groups were respectively composed of 53 controls, 86 patients with gastritis, and 19 patients with gastric cancer. miRNA-181c expression and H. pylori infection were determined by quantitative real-time PCR and PCR, respectively. The subsequent target gene analysis was performed using the bioinformatics approach to understand the possible mechanisms of gastric cancer. We determined significantly lower miRNA-181c expression in the gastric cancer group when compared to the control and gastritis groups, regardless of the presence of H. pylori. There was no difference in miRNA-181c expression between the control group and gastritis group, whether the presence of H. pylori was considered or not. The bioinformatics approach identified several genes as possible targets for miRNA-181c, including the X-linked inhibitor of apoptosis (XIAP) gene (which encodes a protein that belongs to a family of apoptotic suppressor proteins), the caspase 9 gene, and the caspase 3 gene. All target genes identified may be involved in gastric cancer and apoptosis pathways. The results suggest that the presence of H. pylori has no influence on microRNA expression and that the downregulation of miR-181c may play an important role in gastric cancer progression by controlling important genes associated with apoptosis. Therefore, miRNA-181c may be a potential marker of gastric cancer. (AU)

• Processo FAPESP: 15/11613-9 - Análise da Expressão dos Micros RNAs miR146a, miR155 e miR181c e dos genes da Interleucina 2 e Fator de Necrose Tumoral e suas correções com o H. pylori nas doenças gástricas
• Beneficiário: Luanna Munhoz Zabaglia
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/11371-5 - Análise da Expressão de Micro RNAs e de Genes Associados a Apoptose e a Inflamação nas Doenças Gástricas: Uma Possível Relação com o Helicobacter pylori
• Beneficiário: Lucas Trevizani Rasmussen
• Modalidade de apoio: Auxílio à Pesquisa - Regular