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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Decreased MicroRNA miR-181c Expression Associated with Gastric Cancer

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Zabaglia, Luanna Munhoz [1] ; Bartolomeu, Nicole Chiuso [1] ; dos Santos, Monica Pezenatto [1] ; Peruquetti, Rita Luiza [1] ; Chen, Elizabeth [2] ; Cardoso Smith, Marilia de Arruda [2] ; Marques Payao, Spencer Luiz [1] ; Rasmussen, Lucas Trevizani [1]
Total Authors: 8
[1] USC, Proreitoria Pesquisa & Posgrad, Irma Arminda 10-50, BR-17011160 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF GASTROINTESTINAL CANCER; v. 49, n. 1, p. 97-101, MAR 2018.
Web of Science Citations: 9

This study investigated miRNA-181c expression in control patients (healthy gastric mucosa), patients with gastritis, and patients with gastric cancer. The presence of Helicobacter pylori was determined, and the associations between H. pylori infection, levels of miRNA-181c expression, and gastric disease were also analyzed. A total of 158 subjects were included in the study, and the three groups were respectively composed of 53 controls, 86 patients with gastritis, and 19 patients with gastric cancer. miRNA-181c expression and H. pylori infection were determined by quantitative real-time PCR and PCR, respectively. The subsequent target gene analysis was performed using the bioinformatics approach to understand the possible mechanisms of gastric cancer. We determined significantly lower miRNA-181c expression in the gastric cancer group when compared to the control and gastritis groups, regardless of the presence of H. pylori. There was no difference in miRNA-181c expression between the control group and gastritis group, whether the presence of H. pylori was considered or not. The bioinformatics approach identified several genes as possible targets for miRNA-181c, including the X-linked inhibitor of apoptosis (XIAP) gene (which encodes a protein that belongs to a family of apoptotic suppressor proteins), the caspase 9 gene, and the caspase 3 gene. All target genes identified may be involved in gastric cancer and apoptosis pathways. The results suggest that the presence of H. pylori has no influence on microRNA expression and that the downregulation of miR-181c may play an important role in gastric cancer progression by controlling important genes associated with apoptosis. Therefore, miRNA-181c may be a potential marker of gastric cancer. (AU)

FAPESP's process: 15/11613-9 - Analysis of expression of microRNAs MIR146a, miR155 e miR181c, interleukin 2 and tumor necrosis factors and your correlation with the presence of h. pylori and gastric disease
Grantee:Luanna Munhoz Zabaglia
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/11371-5 - Analysis of expression of Micro RNAs and genes associated with apoptosis and chronic inflammation in gastric diseases: a possible relation with Helicobacter pylori
Grantee:Lucas Trevizani Rasmussen
Support type: Regular Research Grants