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Analysis of expression of Micro RNAs and genes associated with apoptosis and chronic inflammation in gastric diseases: a possible relation with Helicobacter pylori

Grant number: 15/11371-5
Support type:Regular Research Grants
Duration: October 01, 2015 - September 30, 2017
Field of knowledge:Biological Sciences - Genetics - Human and Medical Genetics
Principal Investigator:Lucas Trevizani Rasmussen
Grantee:Lucas Trevizani Rasmussen
Home Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Sagrado Coração (USC). Bauru , SP, Brazil
Assoc. researchers:Ana Elizabete Silva ; Jéssica Lima Melchiades ; Juliana Garcia de Oliveira Cucolo ; Luanna Munhoz Zabaglia ; Marilia de Arruda Cardoso Smith ; Spencer Luiz Marques Payão

Abstract

Persistent infection of the gastric mucosa by Helicobacter pylori (H. pylori), promotes a chronic inflammation, providing favorable microenvironment to atrophic gastritis with possible progression to gastric cancer. It is estimated that 50 % of the world population is infected by H. pylori, but the clinical outcome of the infection depends not only on the presence of the bacterium, but also genetic factors of H. pylori and host. CagA and vacA genes are the main virulence markers of H. pylori and may influence the immune response and chronic inflammation, favoring epithelial changes; which in turn, stimulates apoptosis, and the imbalance between cell proliferation and death, main factor related to progression to gastric cancer. Deregulation in the expression of genes involved in this control event can contribute to the development of gastric disorders and changes in gene expression may be influenced by microRNAs. Several studies have shown the importance of miRNAs in tissue homeostasis, as well as their deregulation due to H. pylori infection. In this way, using gastric biopsies of dyspeptic patients and patients with gastric cancer, the purpose of this study are: (I) analyses the gene expression of microRNAs miR-146, miR-155, miR-let-7a e miR-let-7b; (II) analyses the gene expression of mRNA: Smac/Diablo, Caspase 9 e XIAP, TNF-± e IL-2; (III) Correlate the gene expression of microRNAs and mRNA studded; (IV) Correlate the gene expression of microRNAs and mRNA with presence of H. pylori and genes cagA, vacA e dupA. The development of this approach may open new perspectives for the characterization of factors involved in peptic disease, gene regulation, as well as clarification on the interaction among genes involved in gastric diseases, seeking a better understanding of the pathophysiological mechanisms of these diseases. Together these data may suggest biomarkers and groups at risk for progression to gastric cancer. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DOS SANTOS, MONICA PEZENATTO; SALLAS, MAYARA LUCIANA; ZAPPAROLI, DIANA; ORCINI, WILSON APARECIDO; CHEN, ELIZABETH; CARDOSO SMITH, MARILIA DE ARRUDA; MARQUES PAYAO, SPENCER L.; RASMUSSEN, LUCAS TREVIZANI. Lack of Association between IL-6 Polymorphisms and Haplotypes with Gastric Cancer. Journal of Cellular Biochemistry, v. 120, n. 6, p. 9448-9454, JUN 2019. Web of Science Citations: 2.
SALLAS, MAYARA LUCIANA; DOS SANTOS, MONICA PEZENATTO; ORCINI, WILSON APARECIDO; DAVID, ERICA BOARATO; PERUQUETTI, RITA LUIZA; MARQUES PAYAO, SPENCER LUIZ; RASMUSSEN, LUCAS TREVIZANI. Status (on/off) of oipA gene: their associations with gastritis and gastric cancer and geographic origins. Archives of Microbiology, v. 201, n. 1, p. 93-97, JAN 2019. Web of Science Citations: 3.
ZABAGLIA, LUANNA MUNHOZ; SALLAS, MAYARA LUCIANA; DOS SANTOS, MONICA PEZENATTO; ORCINI, WILSON APARECIDO; PERUQUETTI, RITA LUIZA; CONSTANTINO, DULCE HELENA; CHEN, ELIZABETH; CARDOSO SMITH, MARILIA DE ARRUDA; PAYAO, SPENCER-LUIZ MARQUES; RASMUSSEN, LUCAS TREVIZANI. Expression of miRNA-146a, miRNA-155, IL-2, and TNF-alpha in inflammatory response to Helicobacter pylori infection associated with cancer progression. ANNALS OF HUMAN GENETICS, v. 82, n. 3, p. 135-142, MAY 2018. Web of Science Citations: 2.
ZABAGLIA, LUANNA MUNHOZ; BARTOLOMEU, NICOLE CHIUSO; DOS SANTOS, MONICA PEZENATTO; PERUQUETTI, RITA LUIZA; CHEN, ELIZABETH; CARDOSO SMITH, MARILIA DE ARRUDA; MARQUES PAYAO, SPENCER LUIZ; RASMUSSEN, LUCAS TREVIZANI. Decreased MicroRNA miR-181c Expression Associated with Gastric Cancer. JOURNAL OF GASTROINTESTINAL CANCER, v. 49, n. 1, p. 97-101, MAR 2018. Web of Science Citations: 9.
SALLAS, MAYARA LUCIANA; MELCHIADES, JESSICA LIMA; ZABAGLIA, LUANNA MUNHOZ; DO PRADO MORENO, JULIANA RIBEIRO; ORCINI, WILSON APARECIDO; CHEN, ELIZABETH SUCHI; CARDOSO SMITH, MARILIA DE ARRUDA; MARQUES PAYAO, SPENCER LUIZ; RASMUSSEN, LUCAS TREVIZANI. Prevalence of Helicobacter pylori vacA, cagA, dupA and oipA Genotypes in Patients with Gastric Disease. ADVANCES IN MICROBIOLOGY, v. 7, n. 1, p. 1-9, JAN 2017. Web of Science Citations: 2.

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