Gene and Protein Interaction among Components of the Inflammatory, Apoptosis and microRNAs Pathways in Gastric Disease and Gastric Cancer

• Grant number: 18/08481-1
• Support Opportunities: Regular Research Grants
• Duration: August 01, 2018 - October 31, 2020
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Lucas Trevizani Rasmussen
• Grantee: Lucas Trevizani Rasmussen
• Host Institution: Faculdade de Medicina de Marília (FAMEMA). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). Marília , SP, Brazil
• Associated researchers: Elizabeth Suchi Chen ; Marilia de Arruda Cardoso Smith ; Rita Luiza Peruquetti ; Spencer Luiz Marques Payão

Abstract

The persistent infection of the gastric mucosa by Helicobacter pylori (H. pylori), promotes a chronic inflammation, providing a microenvironment favorable for the appearance of atrophic gastritis with possible progression to gastric cancer. The stimulation to the immune and inflammatory response favors epithelial changes; which in turn stimulate apoptosis, the imbalance between the inflammatory process, death and cell proliferation being the main factor related to the progression of atrophic gastritis to gastric cancer. Deregulation in the gene and protein expression of molecules involved in these controlled events may contribute to the evolution of gastric diseases. The aim of this project: (I) evaluate the expression of miR-7, miR-let-7a, miR-25, miR106b, miR125a and miR155 microRNAs ; (II) To evaluate the expression levels (gene and protein) of apoptosis and cytokine regulatory molecules: Interleukin 6, IL-6 Receptor-±, TRAIL, DR4 AND DR5; (III) Correlate the expression of microRNAs, mRNA and proteins previously selected; (IV) To analyze the interaction miRNAs-mRNA-Protein with the presence of H. pylori and the bacterial virulence genes cagA, cagT and vacA. (V) Validate the results with functional assay in cell culture and bioinformatics. The development of this approach may open new perspectives for the characterization of factors involved in peptic diseases, gene regulation, as well as elucidation regarding the interaction between genes involved in gastric diseases, aiming at a better understanding of the pathophysiological mechanisms of these diseases. Together these data may suggest biomarkers and risk groups for progression to gastric cancer. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, JESSICA; SANTOS, MONICA; DELABIO, ROGER; BARBOSA, MONICA; SMITH, MARILIA; PAYAO, SPENCER; RASMUSSEN, LUCAS. Analysis of Gene Expression of miRNA-106b-5p and TRAIL in the Apoptosis Pathway in Gastric Cancer. GENES, v. 11, n. 4, APR 2020. (18/08481-1)

DOS SANTOS, MONICA PEZENATTO; PEREIRA, JESSICA NUNES; DE LABIO, ROGER WILLIAN; CARNEIRO, LILIAN CARLA; PONTES, JAQUELINE CORREIA; BARBOSA, MONICA SANTIAGO; CARDOSO SMITH, MARILIA DE ARRUDA; MARQUES PAYAO, SPENCER LUIZ; RASMUSSEN, LUCAS TREVIZANI. Decrease of miR-125a-5p in Gastritis and Gastric Cancer and Its Possible Association with H. pylori. JOURNAL OF GASTROINTESTINAL CANCER, JUN 2020. (18/02008-2, 18/08481-1)

DOS SANTOS, MONICA PEZENATTO; SALLAS, MAYARA LUCIANA; ZAPPAROLI, DIANA; ORCINI, WILSON APARECIDO; CHEN, ELIZABETH; CARDOSO SMITH, MARILIA DE ARRUDA; MARQUES PAYAO, SPENCER L.; RASMUSSEN, LUCAS TREVIZANI. Lack of Association between IL-6 Polymorphisms and Haplotypes with Gastric Cancer. Journal of Cellular Biochemistry, v. 120, n. 6, p. 9448-9454, JUN 2019. (18/08481-1, 15/11371-5)

SANTOS, M. P.; PEREIRA, J. N.; DELABIO, R. W.; SMITH, M. A. C.; PAYAO, S. L. M.; CARNEIRO, L. C.; BARBOSA, M. S.; RASMUSSEN, L. T.. Increased expression of interleukin-6 gene in gastritis and gastric cancer. Brazilian Journal of Medical and Biological Research, v. 54, n. 7, 2021. (16/25562-0, 18/02008-2, 18/08481-1)

DOS SANTOS, MONICA PEZENATTO; PEREIRA, JESSICA NUNES; DE LABIO, ROGER WILLIAN; CARNEIRO, LILIAN CARLA; PONTES, JAQUELINE CORREIA; BARBOSA, MONICA SANTIAGO; CARDOSO SMITH, MARILIA DE ARRUDA; MARQUES PAYAO, SPENCER LUIZ; RASMUSSEN, LUCAS TREVIZANI. Decrease of miR-125a-5p in Gastritis and Gastric Cancer and Its Possible Association with H. pylori. JOURNAL OF GASTROINTESTINAL CANCER, v. 52, n. 2, p. 6-pg., 2020-06-06. (18/02008-2, 18/08481-1)