| Texto completo | |
| Autor(es): |
Fernandes, Celio J. C.
[1]
;
Bezerra, Fabio
[1]
;
do Carmo, Maiara das D.
[1]
;
Feltran, Georgia S.
[1]
;
Rossi, Mariana C.
[1]
;
da Silva, Rodrigo A.
[1]
;
Padilha, Pedro de M.
[1]
;
Zambuzzi, Willian F.
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] State Univ Sao Paulo, Dept Chem & Biochem, UNESP, Biosci Inst, Campus Botucatu, Botucatu, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Biomedical Materials Research Part A; v. 106, n. 3, p. 839-849, MAR 2018. |
| Citações Web of Science: | 5 |
| Resumo | |
Significant health concerns have been raised by the high levels of Cr and Co ions into whole blood as resulted of corrosion process released from biomedical implants, but very little is known about their biological behavior in governing cell metabolism. Thus, we prompted to address this issue by exploring the effects of CoCr enriched medium on both fibroblast and preosteoblast (pre-Ob) cells. First, we showed there is a significant difference in Co and Cr releasing dependent on engineered surface, it being even more released in dual acid-etching treating surface (named w/DAE) than the machined surfaces (named wo/DAE). Thereafter, we showed CoCr affects pre-osteoblast and fibroblast metabolism by dynamically modulating integrin-based downstream signaling (FAK, Src, Rac1, and Cofilin). Specifically on this matter, we have shown there is dynamic 1-integrin gene activation up 24 h in both preosteoblast and fibroblast. Our analysis showed also that both pre-Ob and fibroblast are important resource of proinflammatory cytokines when responding to CoCr enriched medium. In addition, survival-related signaling pathway was also affected interfering on survival and proliferating signal, mainly affecting CDK2, mapk-Erk and mapk-p38 phosphorylations, while AKT/PKB-related gene remained active. In addition, during cell adhesion PP2A (an important Ser/Thr phosphatase) was inactive in both cell lineages and it seems be a CoCr's molecular fingerprint, regulating specific metabolic pathways involved with cytoskeleton rearrangement. Altogether, our results showed for the first time CoCr affects cellular performance in vitro by modulating integrin activation-based downstream signaling and requiring a reprograming of inflammatory genes activations in vitro. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 839-849, 2018. (AU) | |
| Processo FAPESP: | 14/22689-3 - Sinalização parácrina mediada por microvesículas e proteínas entre células ósseas e endoteliais durante o desenvolvimento e regeneração do tecido ósseo |
| Beneficiário: | Willian Fernando Zambuzzi |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |