Crotamine induces browning of adipose tissue and increases energy expenditure in mice

Autor(es)

Marinovic, Marcelo P. ^[1] ; Campeiro, Joana D. ^[1] ; Lima, Sunamita C. ^[1] ; Rocha, Andrea L. ^[2] ; Nering, Marcela B. ^[1] ; Oliveira, Eduardo B. ^[3] ; Mori, Marcelo A. ^{[2, 4]} ; Hayashi, Mirian A. F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, EPM, Dept Farmacol, Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, UNIFESP, EPM, Dept Biofis, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Bioquim & Imunol, Ribeirao Preto - Brazil

^[4] Univ Estadual Campinas UNICAMP, Dept Bioquim & Biol Tecidual, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 8, MAR 22 2018.
• Citações Web of Science: 1

Resumo

Crotamine, originally isolated from rattlesnake venom, has been extensively studied due to its pleiotropic biological properties, and special attention has been paid to its antitumor activity. However, long-term treatment with crotamine was accompanied by a reduction in animal body weight gain and by increases in glucose tolerance. As cancer is commonly associated with cachexia, to preclude the possible cancer cachexia-like effect of crotamine, herein this polypeptide was administered in healthy wild-type C57/BL6 mice by the oral route daily, for 21 days. Reduced body weight gain, in addition to decreased white adipose tissue (WAT) and increased brown adipose tissue (BAT) mass were observed in healthy animals in the absence of tumor. In addition, we observed improved glucose tolerance and increased insulin sensitivity, accompanied by a reduction of plasma lipid levels and decreased levels of biomarkers of liver damage and kidney disfunctions. Importantly, long-term treatment with crotamine increased the basal metabolic rate in vivo, which was consistent with the increased expression of thermogenic markers in BAT and WAT. Interestingly, cultured brown adipocyte cells induced to differentiation in the presence of crotamine also showed increases in some of these markers and in lipid droplets number and size, indicating increased brown adipocyte maturation. (AU)

• Processo FAPESP: 13/13392-4 - Avaliação do uso dos análogos da crotamina em diagnóstico ou terapia
• Beneficiário: Mirian Akemi Furuie Hayashi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/02413-1 - Validação da crotamina como biomarcador e avaliação do seu potencial uso na terapia de doenças humanas
• Beneficiário: Mirian Akemi Furuie Hayashi
• Modalidade de apoio: Auxílio à Pesquisa - Regular