Palmitoleic acid (16:1n7) increases oxygen consumption, fatty acid oxidation and ATP content in white adipocytes

Autor(es)

Cruz, Maysa M. ^[1] ; Lopes, Andressa B. ^[2] ; Crisma, Amanda R. ^[3] ; de Sa, Roberta C. C. ^[1] ; Kuwabara, Wilson M. T. ^[3] ; Curi, Rui ^{[3, 4]} ; de Andrade, Paula B. M. ^[4] ; Alonso-Vale, Maria I. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Inst Environm Sci Chem & Pharmaceut, Dept Biol Sci, 210 Sao Nicolau St, BR-09913030 Diadema - Brazil

^[2] Univ Fed Espirito Santo, Hlth Sci Ctr, Dept Nursing, Vitoria - Spain

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil

^[4] Cruzeiro do Sul Univ, Inst Phys Act Sci & Sports, Interdisciplinary Postgrad Program Hlth Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LIPIDS IN HEALTH AND DISEASE; v. 17, MAR 20 2018.
• Citações Web of Science: 8

Resumo

Background: We have recently demonstrated that palmitoleic acid (16:1n7) increases lipolysis, glucose uptake and glucose utilization for energy production in white adipose cells. In the present study, we tested the hypothesis that palmitoleic acid modulates bioenergetic activity in white adipocytes. Methods: For this, 3 T3-L1 pre-adipocytes were differentiated into mature adipocytes in the presence (or absence) of palmitic (16:0) or palmitoleic (16:1n7) acid at 100 or 200 mu M. The following parameters were evaluated:lipolysis, lipogenesis, fatty acid (FA) oxidation, ATP content, oxygen consumption, mitochondrial mass, citrate synthase activity and protein content of mitochondrial oxidative phosphorylation (OXPHOS) complexes. Results: Treatment with 16:1n7 during 9 days raised basal and isoproterenol-stimulated lipolysis, FA incorporation into triacylglycerol (TAG), FA oxidation, oxygen consumption, protein expression of subunits representing OXPHOS complex II, III, and V and intracellular ATP content. These effects were not observed in adipocytes treated with 16:0. Conclusions: Palmitoleic acid, by concerted action on lipolysis, Gamma A esterification, mitochondrial Gamma A oxidation, oxygen consumption and ATP content, does enhance white adipocyte energy expenditure and may act as local hormone. (AU)

• Processo FAPESP: 11/51627-8 - Efeito de ácidos graxos sobre a diferenciação e função do tecido adiposo
• Beneficiário: Maria Isabel Cardoso Alonso-Vale
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/51701-3 - Função mitocondrial durante a adipogênese no tecido adiposo branco (TAB): efeito de ácidos graxos
• Beneficiário: Paula Bresciani Martins de Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores