Simultaneous HPLC determination of flavonoids and phenolic acids profile in Pera-Rio orange juice

Autor(es)

Mesquita, E. ^[1] ; Monteiro, M. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Sch Pharmaceut Sci, Dept Food & Nutr, BR-14800903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 106, p. 54-63, APR 2018.
• Citações Web of Science: 7

Resumo

The aim of this study was to develop and validate an HPLC-DAD method to evaluate the phenolic compounds profile of organic and conventional Pera-Rio orange juice. The proposed method was validated for 10 flavonoids and 6 phenolic acids. A wide linear range (0.01-223.4 mu g.g(-1)), good accuracy (79.5-129.2%) and precision (CV <= 3.8%), low limits of detection (1-22 ng.g(-1)) and quantification (0.7-7.4 mu g), and overall ruggedness were attained. Good recovery was achieved for all phenolic compounds after extraction and cleanup. The method was applied to organic and conventional Pera-Rio orange juices from beginning, middle and end of the 2016 harvest. Flavones rutin, nobiletin and tangeretin, and flavanones hesperidin, narirutin and eriocitrin were identified and quantified in all organic and conventional juices. Identity was confirmed by mass spectrometry. Nineteen non-identified phenolic compounds were quantified based on DAD spectra characteristic of the chemical class: 7 cinnamic acid derivatives, 6 flavanones and 6 flavones. The phenolic compounds profile of Pera-Rio orange juices changed during the harvest; levels increased in organic orange juices, and decreased or were about the same in conventional orange juices. Phenolic compounds levels were higher in organic (0.5-1143.7 mg.100 g(-1)) than in conventional orange juices (0.5-689.7 mg 100 g(-1)). PCA differentiated organic from conventional FS and NFC juices, and conventional FCOJ from conventional FS and NFC juices, thus differentiating cultivation and processing. (AU)

• Processo FAPESP: 14/23303-1 - EMU concedido no processo 2013/10138-0: cromatógrafo líquido (HPLC/DAD)
• Beneficiário: Magali Conceição Monteiro da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 13/10138-0 - Impacto das flavanonas cítricas e da limonina na qualidade do suco de laranja
• Beneficiário: Magali Conceição Monteiro da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular