Ultrasonically nebulized distilled water prevents exogenous histamine hyperreactivity in Toxocara canis-infected mice

Autor(es)

Sá-Nunes, A. ; Medeiros, A. I. ; Jamur, M. C. ; Oliver, C. ; Dias-Baruffi, M. ; Faccioli, L. H. ; Bolzoni, R. M. F. ^[7]

• Tipo de documento: Artigo Científico
• Fonte: Inflammation Research; v. 54, n. 6, p. 243-248, jun. 2005.
• Área do conhecimento: Ciências Biológicas - Imunologia
• Assunto(s): Leucotrienos Histamina Mastócitos

Resumo

This study examines the effect of ultrasonically nebulized distilled water inhalation on the systemic histamine hyperreactivity of Toxocara canis-infected mice. Uninfected and T. canis-infected mice received an intravenous sublethal dose of histamine and lethality rates were documented. At 24 days post infection, infected mice received ultrasonically nebulized distilled water inhalation for 1 h. Twenty-four hours later histamine levels were determined in bronchoalveolar lavage fluid as well as histamine lethality and toluidine blue-stained mast cell number in the lung. T. canis-infected mice showed increased lethality after exposure to histamine in comparison to uninfected mice. Ultrasonically nebulized distilled water inhalation prevented histamine-induced lethality and reduced toluidine blue-stained mast cell numbers in the lung. The correlation between decreases in stained mast cells in the lung after ultrasonically nebulized distilled water inhalation and inhibition of histamine-induced lethality in these animals suggests participation of mast cells in the phenomenon and could be helpful in understanding the mechanisms of hyperreactivity during helminth parasite infections. (AU)

• Processo FAPESP: 95/09691-6 - Mecanismos envolvidos no recrutamento de eosinófilos e sua participação no desenvolvimento da hiper-reatividade brônquica
• Beneficiário: Lúcia Helena Faccioli
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores