Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection

Autor(es)

de Almeida Rodolpho, Joice Margareth ^[1] ; Camillo, Luciana ^[1] ; Silva Araujo, Marcio Sobreira ^[2] ; Speziali, Elaine ^[2] ; Coelho-dos-Reis, Jordana Grazziela ^[2] ; Correia, Ricardo de Oliveira ^[1] ; Neris, Debora Meira ^[1] ; Martins-Filho, Olindo Assis ^[2] ; Teixeira-Carvalho, Andrea ^[2] ; Anibal, Fernanda de Freitas ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Carlos UFSCar, Dept Morphol & Pathol, Lab Inflammat & Infect Dis, Sao Carlos, SP - Brazil

^[2] Rene Rachou Inst FIOCRUZ, Integrated Res Grp Biomarkers, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN IMMUNOLOGY; v. 9, JAN 31 2018.
• Citações Web of Science: 2

Resumo

Eosinophils are multifunctional cells that have cytotoxic proinflammatory activities and stimulate CD4(+) T-cells in experimental models of allergy and parasitic infections. Eosinophils, when exposed to antigens, are activated, expressing the CD38/CD69 molecules and exhibited increased expression of major histocompatibility complex (MHC-II), CD80 and CD86, suggesting they play a role upon Toxocara canis antigen stimulation. In the present study, we evaluated the profile of eosinophils using conventional and image flow cytometry upon experimental T. canis infection. T. canis antigens induced a robust activation on this subset, contributing to the immune responses elicited in the experimental model for T. canis-associated visceral larva migrans syndrome. Data analysis demonstrated that, during murine T. canis infection, eosinophils from peripheral blood, spleen, and bone marrow presented upregulated expression of CD69/MHC-II/CD80/CD86. As opposed to splenic and bone marrow eosinophils, circulating eosinophils had increased expression of activation markers upon T. canis infection. The enhanced connectivity between eosinophils and T-cells in T. canis-infected mice in all three compartments (peripheral blood, spleen, and bone marrow) also supports the hypothesis that eosinophils may adopt a role during T. canis infection. Moreover, in vitro T. canis antigen stimulation resulted in activation and upregulation of co-stimulatory-related molecules by bone marrow-derived eosinophils. Our findings are evidence of activation and upregulation of important activation and co-stimulatory-related molecules in eosinophils and suggest a reshape of activation hierarchy toward eosinophils during experimental T. canis infection. (AU)

• Processo FAPESP: 12/08024-3 - Avaliação do papel dos eosinófilos como células apresentadoras de antígenos in vitro e ex vivo
• Beneficiário: Joice Margareth de Almeida Rodolpho
• Modalidade de apoio: Bolsas no Brasil - Doutorado