Inhibition of 5-lipoxygenase attenuates inflammation and bone resorption in lipopolysaccharide-induced periodontal disease

Autor(es)

Lopes, Debora E. M. ^[1] ; Jabr, Camila L. ^[1] ; Dejani, Naiara N. ^[2] ; Saraiva, Amanda C. ^[2] ; de Aquino, Sabrina G. ^{[1, 3]} ; Medeiros, Alexandra I. ^[2] ; Rossa Junior, Carlos ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sch Dent Araraquara, Dept Diag & Surg, Rua Humaita, 1680 Ctr, BR-14801903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Biol Sci, Sao Paulo - Brazil

^[3] Univ Fed Paraiba, Sch Dent, Hlth Sci Ctr, Joao Pessoa, Paraiba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 89, n. 2, p. 235-245, FEB 2018.
• Citações Web of Science: 3

Resumo

BackgroundArachidonate-5-lipoxygenase (5-LO) activity and increased leukotriene B4 (LTB4) production have been implicated in various inflammatory conditions. Increased production of leukotrienes has been associated with periodontal diseases; however, their relative contribution to tissue destruction is unknown. In this study, an orally active specific 5-LO inhibitor is used to assess its role in inflammation and bone resorption in a murine model of lipopolysaccharide (LPS)-induced periodontal disease. MethodsPeriodontal disease was induced in Balb/c mice by direct injections of LPS into the palatal gingival tissues adjacent to the maxillary first molars three times per week for 4weeks. Animals were treated with biochemical inhibitor (2mg/kg/daily) or the same volume of the vehicle by oral gavage. Microcomputed tomography analysis was used to assess bone resorption. Enzyme immunoassay determined LTB4, and enzyme-linked immunosorbent assays quantified tumor necrosis factor (TNF), interleukin (IL)-12, and IL-10 in gingival tissues. Histologic sections were used for the morphometric analysis (number of neutrophils and mononuclear cells). Osteoclasts were counted in tartrate-resistant acid phosphatase-stained sections. ResultsAdministration of 5-LO inhibitor effectively reduced production of LTB4 (23.7% decrease) and significantly reduced TNF and IL-12 levels in gingival tissues. Moreover, reduction of LTB4 levels in gingival tissues was associated with a significant decrease in bone resorption and a marked reduction in number of osteoclasts and inflammatory cells. Conclusion5-LO activity plays a relevant role in inflammation and bone resorption associated with the LPS model of experimental periodontal disease. (AU)

• Processo FAPESP: 11/17611-7 - Efeito da fagocitose de células apoptóticas por células dendríticas na diferenciação de células Th17: função de PGE2
• Beneficiário: Alexandra Ivo de Medeiros
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/23580-0 - Diferenciação de células Th17 durante a fagocitose de células apoptóticas infectadas: determinação das vias de sinalização intracelular via PGE2 e receptores EP
• Beneficiário: Naiara Naiana Dejani
• Modalidade de apoio: Bolsas no Brasil - Doutorado