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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inhibition of 5-lipoxygenase attenuates inflammation and bone resorption in lipopolysaccharide-induced periodontal disease

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Author(s):
Lopes, Debora E. M. [1] ; Jabr, Camila L. [1] ; Dejani, Naiara N. [2] ; Saraiva, Amanda C. [2] ; de Aquino, Sabrina G. [1, 3] ; Medeiros, Alexandra I. [2] ; Rossa Junior, Carlos [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ, Sch Dent Araraquara, Dept Diag & Surg, Rua Humaita, 1680 Ctr, BR-14801903 Araraquara, SP - Brazil
[2] Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Biol Sci, Sao Paulo - Brazil
[3] Univ Fed Paraiba, Sch Dent, Hlth Sci Ctr, Joao Pessoa, Paraiba - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Periodontology; v. 89, n. 2, p. 235-245, FEB 2018.
Web of Science Citations: 3
Abstract

BackgroundArachidonate-5-lipoxygenase (5-LO) activity and increased leukotriene B4 (LTB4) production have been implicated in various inflammatory conditions. Increased production of leukotrienes has been associated with periodontal diseases; however, their relative contribution to tissue destruction is unknown. In this study, an orally active specific 5-LO inhibitor is used to assess its role in inflammation and bone resorption in a murine model of lipopolysaccharide (LPS)-induced periodontal disease. MethodsPeriodontal disease was induced in Balb/c mice by direct injections of LPS into the palatal gingival tissues adjacent to the maxillary first molars three times per week for 4weeks. Animals were treated with biochemical inhibitor (2mg/kg/daily) or the same volume of the vehicle by oral gavage. Microcomputed tomography analysis was used to assess bone resorption. Enzyme immunoassay determined LTB4, and enzyme-linked immunosorbent assays quantified tumor necrosis factor (TNF), interleukin (IL)-12, and IL-10 in gingival tissues. Histologic sections were used for the morphometric analysis (number of neutrophils and mononuclear cells). Osteoclasts were counted in tartrate-resistant acid phosphatase-stained sections. ResultsAdministration of 5-LO inhibitor effectively reduced production of LTB4 (23.7% decrease) and significantly reduced TNF and IL-12 levels in gingival tissues. Moreover, reduction of LTB4 levels in gingival tissues was associated with a significant decrease in bone resorption and a marked reduction in number of osteoclasts and inflammatory cells. Conclusion5-LO activity plays a relevant role in inflammation and bone resorption associated with the LPS model of experimental periodontal disease. (AU)

FAPESP's process: 11/17611-7 - Effect of efferocytosis by dendritic cells on Th17 differentiation: role of Prostaglandin E2
Grantee:Alexandra Ivo de Medeiros
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 12/23580-0 - Differentiation of Th17 cells during phagocytosis of infected apoptotic cells: Determination of intracellular signaling pathways via PGE2 and EP receptors
Grantee:Naiara Naiana Dejani
Support Opportunities: Scholarships in Brazil - Doctorate