Determination of Cephalosporins by UHPLC-DAD Using Molecularly Imprinted Polymers

Autor(es)

Baeza-Fonte, Alen N. ^[1] ; Garces-Lobo, Idenia ^[2] ; Luaces-Alberto, Markel D. ^[2] ; Goncalves, Luis Moreira ^{[3, 4]} ; Sotomayor, Maria D. P. T. ^[5] ; Valdes-Gonzalez, Aristides C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Havana, Inst Sci & Technol Mat, Universitary Lab Characterizat Struct Subst, Calle Zapata & G S-N, Havana 10400 - Cuba

^[2] Univ Havana, Fac Chem, Dept Analyt Chem, Calle Zapata & G S-N, Havana 10400 - Cuba

^[3] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Ave Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil

^[4] Univ Porto FCUP, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE LAQV, Rua Campo Alegre S-N, P-4169007 Oporto - Portugal

^[5] UNESP Univ Estadual Paulista, Inst Quim, Dept Quim Analit, Ave Prof Francisco Degni 55, BR-14800900 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Chromatographic Science; v. 56, n. 2, p. 187-193, FEB 2018.
• Citações Web of Science: 8

Resumo

Molecularly imprinted polymers (MIPs) were synthesized for the determination of cephalosporins (cephazolin, ceftazidime, cefotaxime, ceftriaxone, cefepime and cephalexin) by ultra-high performance liquid chromatography with diode-array detection (UHPLC-DAD). After optimization, MIPs were synthesized using cephazolin as the template, methacrylic acid as the functional monomer, triethylenglycol dimethacrylate as the crosslinker, acetonitrile/dimethylsulfoxide as porogens and benzoyl peroxide as the radical initiator. Not only this is a novel route of MIP synthesis for cephalosporins, but also this choice of analytes is unique. Chromatographic separation was performed in a C-8 column using a binary gradient with trifluoroacetic acid 0.1% in water and acetonitrile. Linearity was assessed up to 100 mu g mL(-1) and linear correlation coefficients (r) were all higher than 0.99, limits of detection were within the range of 3-12 ng mL(-1), and recoveries from 86 to 102% were obtained for concentrations between 0.05 and 1.0 mu g mL(-1). (AU)

• Processo FAPESP: 16/06926-0 - Desenvolvimento e aplicação de novos sensores químicos nanoestruturados e aplicação de novos sensores químicos nanoestruturados baseados em polímeros biomiméticos
• Beneficiário: Maria Del Pilar Taboada Sotomayor
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional