| Full text | |
| Author(s): |
Baeza-Fonte, Alen N.
[1]
;
Garces-Lobo, Idenia
[2]
;
Luaces-Alberto, Markel D.
[2]
;
Goncalves, Luis Moreira
[3, 4]
;
Sotomayor, Maria D. P. T.
[5]
;
Valdes-Gonzalez, Aristides C.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Havana, Inst Sci & Technol Mat, Universitary Lab Characterizat Struct Subst, Calle Zapata & G S-N, Havana 10400 - Cuba
[2] Univ Havana, Fac Chem, Dept Analyt Chem, Calle Zapata & G S-N, Havana 10400 - Cuba
[3] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Ave Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[4] Univ Porto FCUP, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE LAQV, Rua Campo Alegre S-N, P-4169007 Oporto - Portugal
[5] UNESP Univ Estadual Paulista, Inst Quim, Dept Quim Analit, Ave Prof Francisco Degni 55, BR-14800900 Araraquara, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Journal of Chromatographic Science; v. 56, n. 2, p. 187-193, FEB 2018. |
| Web of Science Citations: | 8 |
| Abstract | |
Molecularly imprinted polymers (MIPs) were synthesized for the determination of cephalosporins (cephazolin, ceftazidime, cefotaxime, ceftriaxone, cefepime and cephalexin) by ultra-high performance liquid chromatography with diode-array detection (UHPLC-DAD). After optimization, MIPs were synthesized using cephazolin as the template, methacrylic acid as the functional monomer, triethylenglycol dimethacrylate as the crosslinker, acetonitrile/dimethylsulfoxide as porogens and benzoyl peroxide as the radical initiator. Not only this is a novel route of MIP synthesis for cephalosporins, but also this choice of analytes is unique. Chromatographic separation was performed in a C-8 column using a binary gradient with trifluoroacetic acid 0.1% in water and acetonitrile. Linearity was assessed up to 100 mu g mL(-1) and linear correlation coefficients (r) were all higher than 0.99, limits of detection were within the range of 3-12 ng mL(-1), and recoveries from 86 to 102% were obtained for concentrations between 0.05 and 1.0 mu g mL(-1). (AU) | |
| FAPESP's process: | 16/06926-0 - Development and application of new nanostructured chemical sensors and applications of new nanostructured chemical sensors based on biomimetic polymers |
| Grantee: | Maria Del Pilar Taboada Sotomayor |
| Support Opportunities: | Research Grants - Visiting Researcher Grant - International |