Antimicrobial activity of denture adhesive associated with Equisetum giganteum- and Punica granatum-enriched fractions against Candida albicans biofilms on acrylic resin surfaces

Autor(es)

Martins Almeida, Nara Ligia ^[1] ; Saldanha, Luiz Leonardo ^{[2, 3]} ; da Silva, Rafaela Alves ^[1] ; Pinke, Karen Henriette ^[1] ; da Costa, Eliane Ferraz ^[1] ; Porto, Vinicius Carvalho ^[4] ; Dokkedal, Anne Ligia ^[2] ; Lara, Vanessa Soares ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Surg Stomatol Pathol & Radiol, Bauru - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Biol Sci, Fac Sci, Bauru - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Bot, Botucatu, SP - Brazil

^[4] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont & Periodont, Bauru - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOFOULING; v. 34, n. 1, p. 62-73, 2018.
• Citações Web of Science: 0

Resumo

Candida biofilms adhere to the internal surface of removable dentures, which is an etiological factor in the pathogenesis of denture stomatitis (DS). Adhesive materials are used at the base of maxillary complete dentures to improve their retention and chewing qualities. This article reports the antimicrobial activity of the enriched fractions of Equisetum giganteum and Punica granatum incorporated into a denture adhesive against C. albicans biofilm. The biofilms were induced on the surface of heat-cured acrylic resin specimens that were previously treated with a mixture of adhesive/herb extracts. The antimicrobial activity was evaluated by CFU counts, XTT reduction, and SEM and CLSM analysis. Both herb extracts amplified the anti-biofilm action of the adhesive on the acrylic resin by up to 12h. Therefore, when these extracts were combined with COREGA (R), they played a collaborative and innovative role in biofilm control and can be considered alternatives for temporary use in the treatment and/or prevention of DS. (AU)

• Processo FAPESP: 12/12458-9 - Fitoterápico Equisetum giganteum e estomatite protética: estudo da ação antimicrobiana, antiaderente e antiinflamatória contra Candida albicans, e potencial citotóxico sobre células epiteliais do palato humano
• Beneficiário: Vanessa Soares Lara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/07012-7 - Equisetum giganteum e Punica granatum associados ao adesivo fixador de próteses totais Ultra Corega: atividade antimicrobiana e antiaderente contra biofilmes induzidos de Candida albicans, e potencial citotóxico sobre fibroblastos gengivais humanos
• Beneficiário: Nara Ligia Martins Almeida
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/03965-2 - Papel do sistema renina-angiotensina em diferentes modelos inflamatórios orais: uma abordagem interdisciplinar experimental e clínica
• Beneficiário: Carlos Ferreira dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Temático