Antimicrobial activity of denture adhesive associated with Equisetum giganteum- and Punica granatum-enriched fractions against Candida albicans biofilms on acrylic resin surfaces

Author(s)

Martins Almeida, Nara Ligia ^[1] ; Saldanha, Luiz Leonardo ^{[2, 3]} ; da Silva, Rafaela Alves ^[1] ; Pinke, Karen Henriette ^[1] ; da Costa, Eliane Ferraz ^[1] ; Porto, Vinicius Carvalho ^[4] ; Dokkedal, Anne Ligia ^[2] ; Lara, Vanessa Soares ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Surg Stomatol Pathol & Radiol, Bauru - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Biol Sci, Fac Sci, Bauru - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Bot, Botucatu, SP - Brazil

^[4] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont & Periodont, Bauru - Brazil

• Document type: Journal article
• Source: BIOFOULING; v. 34, n. 1, p. 62-73, 2018.
• Web of Science Citations: 0

Abstract

Candida biofilms adhere to the internal surface of removable dentures, which is an etiological factor in the pathogenesis of denture stomatitis (DS). Adhesive materials are used at the base of maxillary complete dentures to improve their retention and chewing qualities. This article reports the antimicrobial activity of the enriched fractions of Equisetum giganteum and Punica granatum incorporated into a denture adhesive against C. albicans biofilm. The biofilms were induced on the surface of heat-cured acrylic resin specimens that were previously treated with a mixture of adhesive/herb extracts. The antimicrobial activity was evaluated by CFU counts, XTT reduction, and SEM and CLSM analysis. Both herb extracts amplified the anti-biofilm action of the adhesive on the acrylic resin by up to 12h. Therefore, when these extracts were combined with COREGA (R), they played a collaborative and innovative role in biofilm control and can be considered alternatives for temporary use in the treatment and/or prevention of DS. (AU)

• FAPESP's process: 12/12458-9 - Equisetum giganteum phytomedicine and denture stomatitis: study of antimicrobial, nonstick and anti-inflammatory action against Candida albicans, and cytotoxic potential in human palatal epithelial cell
• Grantee: Vanessa Soares Lara
• Support type: Regular Research Grants
• FAPESP's process: 14/07012-7 - Equisetum giganteum and Punica granatum associated with denture adhesive Corega Ultra: antimicrobial and anti-adherent activities against induced biofilms of Candida albicans and cytotoxic potential in human gingival fibroblastos
• Grantee: Nara Ligia Martins Almeida
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach
• Grantee: Carlos Ferreira dos Santos
• Support type: Research Projects - Thematic Grants