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Equisetum giganteum and Punica granatum associated with denture adhesive Corega Ultra: antimicrobial and anti-adherent activities against induced biofilms of Candida albicans and cytotoxic potential in human gingival fibroblastos

Grant number: 14/07012-7
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2014
Effective date (End): February 29, 2016
Field of knowledge:Health Sciences - Dentistry
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Vanessa Soares Lara
Grantee:Nara Ligia Martins Almeida
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


Denture adhesives currently represent a valuable contrivance for obtaining the retention, stability and masticatory function and thus improve chewing ability and reduce the amount of particles retained in the denture and assist in the distribution of occlusal forces food, thus minimizing the points of location and providing a better quality of life for patients with Total Dentures (TD). Many of these patients presents Denture Stomatitis (DP), a multifactorial inflammatory disease closely related to the immunosuppression state and by the colonization of the fungus Candida albicans over the acrylic resin of the TDs, in the form of biofilms highly resistant to conventional treatments. This becomes extremely important to find alternative antimicrobial therapies and without deleterious effects to the patients, as traditional drugs. The phytomedicines fits in this context, where in addition of the characteristics mentioned above, are less costly than synthetic drugs. Thus, denture adhesives associated with phytomedicines may presents benefits to prevent the adhesion and/or help to fight the main microorganism involved with the DS, the fungus C. albicans, in addition to the inherent benefits of the adhesive itself. The purpose of this study is to evaluate the action of Equisetum giganteum and Punica granatum Linné phytomedicine against associated with the denture adhesive Corega Ultra® on biofilm of C. albicans. For that, the antimicrobial activity of the phytomedicines will be evaluated by in vitro tests against fungus C. albicans through the Minimum Inhibitory Concentration (MIC) and the study of fungal viability on the induced biofilm formed on the thermopolimeryzed acrylic resin by the colorimetric XTT assay will be evaluated. Furthermore, it will be also evaluated the cytotoxicity of the extracts and of the adhesive over the human gingival fibroblasts in vitro by colorimetric MTT assay. The expression of the results and the statistical tests will be applied according to the presence or absence of normality in the evaluations (p<0.05). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARTINS ALMEIDA, NARA LIGIA; SALDANHA, LUIZ LEONARDO; DA SILVA, RAFAELA ALVES; PINKE, KAREN HENRIETTE; DA COSTA, ELIANE FERRAZ; PORTO, VINICIUS CARVALHO; DOKKEDAL, ANNE LIGIA; LARA, VANESSA SOARES. Antimicrobial activity of denture adhesive associated with Equisetum giganteum- and Punica granatum-enriched fractions against Candida albicans biofilms on acrylic resin surfaces. BIOFOULING, v. 34, n. 1, p. 62-73, . (12/12458-9, 14/07012-7, 15/03965-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ALMEIDA, Nara Ligia Martins. Equisetum giganteum and Punica granatum Linn associated with denture adhesive: antimicrobial activity against Candida albicans biofilms. 2016. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.

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