Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts

Autor(es):Mostrar menos -

Bogliotti, Yanina Soledad ^[1] ; Wu, Jun ^{[2, 3, 4, 5]} ; Vilarino, Marcela ^[1] ; Okamura, Daiji ^{[5, 6]} ; Soto, Delia Alba ^[1] ; Zhong, Cuiqing ^[2] ; Sakurai, Masahiro ^{[2, 3, 4, 5]} ; Sampaio, Rafael Vilar ^[1] ; Suzuki, Keiichiro ^[2] ; Belmonte, Juan Carlos Izpisua ^[2] ; Ross, Pablo Juan ^[1]

Afiliação do(s) autor(es)

^[1] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 - USA

^[2] Salk Inst Biol Studies, Gene Express Lab, La Jolla, CA 92037 - USA

^[3] Univ Texas Southwestern Med Ctr Dallas, Dept Mol Biol, Dallas, TX 75390 - USA

^[4] Univ Texas Southwestern Med Ctr Dallas, Hamon Ctr Regenerat Sci & Med, Dallas, TX 75390 - USA

^[5] Univ Catolica San Antonio Murcia, Murcia 30107 - Spain

^[6] Kindai Univ, Grad Sch Agr, Dept Adv Biosci, Nara 6318505 - Japan

• Tipo de documento: Artigo Científico
• Fonte: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA; v. 115, n. 9, p. 2090-2095, FEB 27 2018.
• Citações Web of Science: 27

Resumo

Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and biomedical perspectives, the derivation of stable ESCs from domestic ungulates is important for genomic testing and selection, genome engineering, and modeling human diseases. Cattle are one of the most important domestic ungulates that are commonly used for food and bioreactors. To date, however, it remains a challenge to produce stable pluripotent bovine ESC lines. Employing a culture system containing fibroblast growth factor 2 and an inhibitor of the canonical Wnt-signaling pathway, we derived pluripotent bovine ESCs (bESCs) with stable morphology, transcriptome, karyotype, population-doubling time, pluripotency marker gene expression, and epigenetic features. Under this condition bESC lines were efficiently derived (100% in optimal conditions), were established quickly (3-4 wk), and were simple to propagate (by trypsin treatment). When used as donors for nuclear transfer, bESCs produced normal blastocyst rates, thereby opening the possibility for genomic selection, genome editing, and production of cattle with high genetic value. (AU)

• Processo FAPESP: 15/25111-5 - Análise e modulação da metilação da H3K9 em células somáticas de bovinos
• Beneficiário: Rafael Vilar Sampaio
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs