Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil

Autor(es):Mostrar menos -

Marques Rossi, Gabriel Augusto ^[1] ; Silva, Higor Oliveira ^[1] ; Gamero Aguilar, Carlos Eduardo ^[1] ; Rochetti, Arina Lazaro ^[2] ; Pascoe, Ben ^[3] ; Meric, Guillaume ^[3] ; Mourkas, Evangelos ^[3] ; Hitchings, Matthew D. ^[4] ; Mathias, Luis Antonio ^[1] ; de Azevedo Ruiz, Vera Letticie ^[2] ; Fukumasu, Heidge ^[2] ; Sheppard, Samuel K. ^[3] ; Centola Vidal, Ana Maria ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Dept Med Vet Prevent & Reprod Anim, FCAV, Via Acesso Paulo Castellane S-N, BR-14884900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Med Vet, FZEA, Ave Duque de Caxias Norte 225, BR-13635900 Sao Paulo - Brazil

^[3] Univ Bath, Dept Biol & Biochem, Milner Ctr Evolut, 4 South, Lab 0-39, Bath BA2 7AY, Avon - England

^[4] Swansea Univ, Med Sch, Swansea SA2 8PP, W Glam - Wales

• Tipo de documento: Artigo Científico
• Fonte: FEMS Microbiology Letters; v. 365, n. 3 FEB 2018.
• Citações Web of Science: 2

Resumo

The genomes of 262 Bacillus cereus isolates were analyzed including 69 isolates sampled from equipment, raw milk and dairy products from Brazil. The population structure of isolates showed strains belonging to known phylogenetic groups II, III, IV, V and VI. Almost all the isolates obtained from dairy products belonged to group III. Investigation of specific alleles revealed high numbers of isolates carrying toxin-associated genes including cytK (53.62%), hblA (59.42%), hblC (44.93%), hblD ( 53.62%), nheA (84.06%), nheB (89.86%) and nheC (84.06%) with isolates belonging to groups IV and V having significant higher prevalence of hblACD and group IV of CytK genes. Strains from dairy products had significantly lower prevalence of CytK and hblACD genes compared to isolates from equipment and raw milk/bulk tanks. Genes related to sucrose metabolism were detected at higher frequency in isolates obtained from raw milk compared to strains from equipment and utensils. The population genomic analysis demonstrated the diversity of strains and variability of putative function among B. cereus group isolates in Brazilian dairy production, with large numbers of strains potentially able to cause foodborne illness. This detailed information will contribute to targeted interventions to reduce milk contamination and spoilage associated with B. cereus in Brazil. (AU)

• Processo FAPESP: 14/13104-1 - Expressão dos genes relacionados com a produção de toxinas gastroentéricas de Bacillus cereus durante a shelf-life de leite pasteurizado produzido com matéria prima previamente contaminada
• Beneficiário: Gabriel Augusto Marques Rossi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/19214-9 - Genômica comparativa e identificação de fatores envolvidos na sobrevivência de Bacillus cereus na cadeia produtiva leiteira
• Beneficiário: Gabriel Augusto Marques Rossi
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/20874-0 - Impacto do B. cereus na cadeia produtiva leiteira: Avaliação da expressão gênica ligada a produção de toxinas gastroentericas e enzimas deteriorantes durante a vida de prateleira de leite pasteurizado e determinação da eficácia de desinfetantes sobre biof
• Beneficiário: Ana Maria Centola Vidal
• Modalidade de apoio: Auxílio à Pesquisa - Regular