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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil

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Author(s):
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Marques Rossi, Gabriel Augusto [1] ; Silva, Higor Oliveira [1] ; Gamero Aguilar, Carlos Eduardo [1] ; Rochetti, Arina Lazaro [2] ; Pascoe, Ben [3] ; Meric, Guillaume [3] ; Mourkas, Evangelos [3] ; Hitchings, Matthew D. [4] ; Mathias, Luis Antonio [1] ; de Azevedo Ruiz, Vera Letticie [2] ; Fukumasu, Heidge [2] ; Sheppard, Samuel K. [3] ; Centola Vidal, Ana Maria [2]
Total Authors: 13
Affiliation:
[1] Univ Estadual Paulista, UNESP, Dept Med Vet Prevent & Reprod Anim, FCAV, Via Acesso Paulo Castellane S-N, BR-14884900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Med Vet, FZEA, Ave Duque de Caxias Norte 225, BR-13635900 Sao Paulo - Brazil
[3] Univ Bath, Dept Biol & Biochem, Milner Ctr Evolut, 4 South, Lab 0-39, Bath BA2 7AY, Avon - England
[4] Swansea Univ, Med Sch, Swansea SA2 8PP, W Glam - Wales
Total Affiliations: 4
Document type: Journal article
Source: FEMS Microbiology Letters; v. 365, n. 3 FEB 2018.
Web of Science Citations: 2
Abstract

The genomes of 262 Bacillus cereus isolates were analyzed including 69 isolates sampled from equipment, raw milk and dairy products from Brazil. The population structure of isolates showed strains belonging to known phylogenetic groups II, III, IV, V and VI. Almost all the isolates obtained from dairy products belonged to group III. Investigation of specific alleles revealed high numbers of isolates carrying toxin-associated genes including cytK (53.62%), hblA (59.42%), hblC (44.93%), hblD ( 53.62%), nheA (84.06%), nheB (89.86%) and nheC (84.06%) with isolates belonging to groups IV and V having significant higher prevalence of hblACD and group IV of CytK genes. Strains from dairy products had significantly lower prevalence of CytK and hblACD genes compared to isolates from equipment and raw milk/bulk tanks. Genes related to sucrose metabolism were detected at higher frequency in isolates obtained from raw milk compared to strains from equipment and utensils. The population genomic analysis demonstrated the diversity of strains and variability of putative function among B. cereus group isolates in Brazilian dairy production, with large numbers of strains potentially able to cause foodborne illness. This detailed information will contribute to targeted interventions to reduce milk contamination and spoilage associated with B. cereus in Brazil. (AU)

FAPESP's process: 15/20874-0 - Bacillus cereus impact in dairy supply chain: gene expression related to gastroenteric toxins, spoilage enzymes during the shelf-life of pasteurized milk and determination of disinfectants efficacy on biofilms
Grantee:Ana Maria Centola Vidal
Support type: Regular Research Grants
FAPESP's process: 14/13104-1 - Gene expression related to the production of Bacillus cereus gastroenteric toxins during the shelf-life of pasteurized milk produced with contaminated milk
Grantee:Gabriel Augusto Marques Rossi
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/19214-9 - Comparative genomics and identification of factors involved in Bacillus cereus survival through the dairy supply chain
Grantee:Gabriel Augusto Marques Rossi
Support type: Scholarships abroad - Research Internship - Doctorate