| Texto completo | |
| Autor(es): |
de Lima, Aline D.
[1]
;
Guido, Maria C.
[1]
;
Tavares, Elaine R.
[1]
;
Carvalho, Priscila O.
[1]
;
Marques, Alyne F.
[1]
;
de Melo, Marcelo D. T.
[2, 3]
;
Salemi, Vera M. C.
[2, 3]
;
Kalil-Filho, Roberto
[2, 3]
;
Maranhao, Raul C.
[1, 4]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Med Sch Hosp, Lab Metab & Lipids, Heart Inst InCor, Lo SS, Av Dr Eneas de Carvalho Aguiar 44, Bl 2, BR-05403900 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Lo SS, Heart Failure Unit, Av Dr Eneas de Carvalho Aguiar 44, Bl 2, BR-05403900 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Lo SS, Heart Inst InCor, Clin Cardiol Div, Av Dr Eneas de Carvalho Aguiar 44, Bl 2, BR-05403900 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Fac Pharmaceut Sci, Lo SS, Av Dr Eneas de Carvalho Aguiar 44, Bl 2, BR-05403900 Sao Paulo, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | LIPIDS; v. 53, n. 2, p. 177-187, FEB 2018. |
| Citações Web of Science: | 1 |
| Resumo | |
Left ventricular (LV) remodeling after myocardial infarction constitutes the structural basis for ventricular dysfunction and heart failure. The characterization underlying the expression of lipoprotein receptors in cardiac dysfunction is scarcely explored. The aim of this study was to analyze the status of lipoprotein receptors on the infarcted and noninfarcted areas of LV and to verify whether nano particles that mimic the lipid structure of low-density lipoprotein (LDL) and have the ability to bind to LDL receptors (LDE) are taken up more avidly by the noninfarcted LV. 13 male Wistar rats with left coronary artery ligation (myocardial infarction {[}MI]) and 12 animals with SHAM operation (SHAM) were used in this study. 6 weeks after the procedure, the quantification of low-density lipoprotein receptor (LDLR), LDL receptor-related protein 1 (LRP1), scavenger receptor-class B type I (SR-BI) lipoprotein receptors, and PCNA proliferation marker, and tissue uptake of radioactively labeled LDE were performed. Immunohistochemistry and Western blot analysis showed that LDLR, LRP1, SR-BI, and PCNA, expression in infarcted area of MI was remarkably higher than SHAM and noninfarcted subendocardial (SEN) and interstitial (INT) areas. In addition, in SEN noninfarcted area of MI, the presence of LDLR was about threefold higher than in SHAM SEN and INT noninfarcted areas. The LDE uptake of noninfarcted LV of MI group was about 30% greater than that of SHAM group. In conclusion, these findings regarding the status of lipoprotein receptors after MI induction could help to establish mechanisms on myocardial repairing. In conclusion, infarcted rats with LV dysfunction showed increased expression of lipoprotein receptors mainly in the infarcted area. (AU) | |
| Processo FAPESP: | 14/03742-0 - Projeto temático em medicina translacional: nanopartículas que se ligam a receptores de lipoproteínas no tratamento da aterosclerose, do infarto agudo de miocárdio, do pós-transplante de coração, do câncer e da endometriose |
| Beneficiário: | Raul Cavalcante Maranhao |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |