Molecular analyses of two bacterial sampling methods in ligature-induced periodontitis in rats

Autor(es)

Fontana, Carla Raquel ^[1] ; Grecco, Clovis ^[2] ; Bagnato, Vanderlei Salvador ^[2] ; de Freitas, Laura Marise ^[1] ; Boussios, Constantinos I. ^[3] ; Soukos, Nikolaos S. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Fac Ciencias Farmaceut, Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Inst Fis, Grp Opt, Sao Paulo, SP - Brazil

^[3] MIT, Lab Informat & Decis Syst, Cambridge, MA 02139 - USA

^[4] 111 Dana Res Ctr, Dept Phys, Boston, MA - USA

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL AND EXPERIMENTAL DENTAL RESEARCH; v. 4, n. 1, p. 19-24, FEB 2018.
• Citações Web of Science: 1

Resumo

The prevalence profile of periodontal pathogens in dental plaque can vary as a function of the detection method; however, the sampling technique may also play a role in determining dental plaque microbial profiles. We sought to determine the bacterial composition comparing two sampling methods, one well stablished and a new one proposed here. In this study, a ligature-induced periodontitis model was used in 30 rats. Twenty-seven days later, ligatures were removed and microbiological samples were obtained directly from the ligatures as well as from the periodontal pockets using absorbent paper points. Microbial analysis was performed using DNA probes to a panel of 40 periodontal species in the checkerboard assay. The bacterial composition patterns were similar for both sampling methods. However, detection levels for all species were markedly higher for ligatures compared with paper points. Ligature samples provided more bacterial counts than paper points, suggesting that the technique for induction of periodontitis could also be applied for sampling in rats. Our findings may be helpful in designing studies of induced periodontal disease-associated microbiota. (AU)

• Processo FAPESP: 16/05345-4 - Otimização da terapia fotodinâmica para doenças infecciosas utilizando o peptídeo aureína 1.2
• Beneficiário: Carla Raquel Fontana
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs