Bacterial Nanocellulose Loaded with Bromelain: Assessment of Antimicrobial, Antioxidant and Physical-Chemical Properties

Autor(es)

Ataide, Janaina Artem ^[1] ; de Carvalho, Nathalia Mendes ^[2] ; Rebelo, Marcia de Araujo ^[3] ; Chaud, Marco Vinicius ^[3] ; Grotto, Denise ^[2] ; Gerenutti, Marli ^[2] ; Rai, Mahendra ^[4] ; Mazzola, Priscila Gava ^[5] ; Jozala, Angela Faustino ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Med Sci, Grad Program Med Sci, Campinas, SP - Brazil

^[2] Univ Sorocaba, Dept Technol & Environm Proc, Sorocaba, SP - Brazil

^[3] Univ Sorocaba, LaBNUS, Biomat & Nanotechnol Lab, Sorocaba, SP - Brazil

^[4] SGB Amravati Univ, Dept Biotechnol, Nanobiotechnol Lab, Amravati, Maharashtra - India

^[5] Univ Estadual Campinas, Fac Pharmaceut Sci, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 7, DEC 21 2017.
• Citações Web of Science: 7

Resumo

Bacterial nanocellulose (BNC) has desirable properties for wound healing such as high purity, good shape retention, and high water binding capacity. Bromelain is a protease found in pineapple tissues and has been applied in several fields, it has anti-inflammatory and anticancer properties, promotes cell apoptosis, amongst others. In this work, a BNC based device for the controlled release of bromelain was developed. BNC were submersed in sterilized bromelain solution and incubated at 25 degrees C under 100 rpm for 24 h. Physical-chemical properties, protein concentration, antioxidant and antimicrobial activities were measured. Results demonstrate that BNC could improve bromelain antimicrobial activity 9 times. Those findings allow concluding that bromelain is a promising molecule to be incorporated into BNC's. The BNC's characteristics seem to represent a new promising delivery system of the loaded biomolecule, and protected from external actions. (AU)

• Processo FAPESP: 11/23076-7 - Extração de bromelina a partir de resíduos de abacaxi (Ananas comosus) por precipitação em etanol e avaliação de sua aplicação em hidrogel polimérico
• Beneficiário: Janaína Artem Ataide
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/05930-4 - Desenvolvimento de uma Plataforma para a Produção e Purificação de Bioprodutos
• Beneficiário: Angela Faustino Jozala
• Modalidade de apoio: Auxílio à Pesquisa - Regular