Performance of Different Immobilized Lipases in the Syntheses of Short- and Long-Chain Carboxylic Acid Esters by Esterification Reactions in Organic Media

Autor(es)

de Lima, Lionete Nunes ^[1] ; Mendes, Adriano Aguiar ^[2] ; Fernandez-Lafuente, Roberto ^[3] ; Tardioli, Paulo Waldir ^[1] ; Camargo Giordano, Raquel de Lima ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Chem Engn, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Alfenas, Inst Chem, BR-37130001 Alfenas, MG - Brazil

^[3] CSIC, ICP, Dept Biocatalysts, Campus UAM CSIC, Madrid 28049 - Spain

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 23, n. 4 APR 2018.
• Citações Web of Science: 3

Resumo

Short-chain alkyl esters and sugar esters are widely used in the food, pharmaceutical and cosmetic industries due to their flavor and emulsifying characteristics, respectively. Both compounds can be synthesized via biocatalysis using lipases. This work aims to compare the performance of commercial lipases covalently attached to dry acrylic beads functionalized with oxirane groups (lipases from Candida antarctica type B-IMMCALB-T2-350, Pseudomonas fluorescens-IMMAPF-T2-150, and Thermomyces lanuginosus-IMMTLL-T2-150) and a home-made biocatalyst (lipase from Pseudomonas fluorescens adsorbed onto silica coated with octyl groups, named PFL-octyl-silica) in the syntheses of short-and long-chain carboxylic acid esters. Esters with flavor properties were synthetized by esterification of acetic and butyl acids with several alcohols (e.g., ethanol, 1-butanol, 1-hexanol, and isoamyl alcohol), and sugar esters were synthetized by esterification of oleic and lauric acids with fructose and lactose. All biocatalysts showed similar performance in the syntheses of short-chain alkyl esters, with conversions ranging from 88.9 to 98.4%. However, in the syntheses of sugar esters the performance of PFL-octyl-silica was almost always lower than the commercial IMMCALB-T2-350, whose conversion was up to 96% in the synthesis of fructose oleate. Both biocatalysts showed high operational stability in organic media, thus having great potential for biotransformations. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 11/23194-0 - Síntese enzimática de ésteres de ácidos graxos com aquecimento assistido por micro-ondas
• Beneficiário: Paulo Waldir Tardioli
• Modalidade de apoio: Auxílio à Pesquisa - Regular