| Texto completo | |
| Autor(es): |
Amorim, Fernanda Gobbi
[1, 2]
;
Costa, Tassia Rafaela
[1]
;
Baiwir, Dominique
[3]
;
De Pauw, Edwin
[4]
;
Quinton, Loic
[4]
;
Sampaio, Suely Vilela
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Lab Toxinol, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ave Cafe S-No Campus Univ, BR-14040903 Ribeirao Preto, SP - Brazil
[3] Univ Liege, GIGA Inst, GIGA Prote Facil, B-4000 Liege - Belgium
[4] Univ Liege, Dept Chem, Lab Mass Spectrometry, B-4000 Liege - Belgium
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | TOXINS; v. 10, n. 5 MAY 2018. |
| Citações Web of Science: | 5 |
| Resumo | |
Venom composition varies across snakes from all taxonomic levels and is influenced by the snakes' age, habitat, diet, and sexual dimorphism. The present study reports the first in-depth investigation of venom composition in male and female Bothrops moojeni (B. moojeni) snakes (BmooM and BmooF, respectively) through three proteomics approaches associated with functional, cytotoxic, and immunoreactivity characterization. Compared with BmooM venom, BmooF venom exhibited weaker hyaluronidase, metalloproteinase, and phospholipase activity; stronger recognition by anti-bothropic serum; 1.4-fold stronger cytotoxicity; and greater number of peptides. The increased L-amino acid oxidase expression probably accounted for the stronger immunoreactivity and cytotoxicity of BmooF venom. BmooF and BmooM venom shared only 19% peptides. Some venom components were gender-specific, such as phospholipases B, phospholipase inhibitor, and hyaluronidases in BmooM, and cysteine-rich secretory proteins in BmooF. In conclusion, we describe herein the first proteomics study of B. moojeni snake venom and an in-depth characterization of gender-specific differences in venom composition. Altogether, our findings not only stress the importance of considering the snake's gender during antivenom production, but also help to identify new potential drugs and biotechnological tools. (AU) | |
| Processo FAPESP: | 15/00740-0 - Avaliação do potencial terapêutico de L-aminoácido oxidases isoladas de peçonhas de serpentes como antitumoral: Estudos de genotoxicidade e expressão gênica |
| Beneficiário: | Tássia Rafaella Costa |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 15/26609-7 - Venômica da serpente Bothrops moojeni: Aplicação de toxinas de interesse biotecnológico obtidas por técnicas ômicas |
| Beneficiário: | Fernanda Gobbi Amorim |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 16/20641-9 - Integração da abordagens ômicas para obter o perfil da peçonha da serpente Bothrops moojeni: revelando novas toxinas e divergências ontogênicas |
| Beneficiário: | Fernanda Gobbi Amorim |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |
| Processo FAPESP: | 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular |
| Beneficiário: | Suely Vilela |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |