Microbial functional responses to long-term anthropogenic impact in mangrove soils

Autor(es)

Cabral, Lucelia ^[1] ; Pereira de Sousa, Sanderson Tarciso ^{[2, 1]} ; Lacerda Junior, Gileno Vieira ^{[2, 1]} ; Hawley, Erik ^[3] ; Andreote, Fernando Dini ^[4] ; Hess, Matthias ^[5] ; de Oliveira, Valeria Maia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Res Ctr Chem Biol & Agr CPQBA, Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Campinas, SP - Brazil

^[3] Washington State Univ, Pullman, WA 99164 - USA

^[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Soil Sci, Piracicaba, SP - Brazil

^[5] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 - USA

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 160, p. 231-239, SEP 30 2018.
• Citações Web of Science: 8

Resumo

Mangroves are coastal ecosystems of transition between terrestrial and marine environments, that have been particularly contaminated in the last decades. Organic compounds are part of these contaminants, which have increased in the environment due to industrial activities and accidental oil spills. These contaminants are toxic to higher organisms, but microorganisms can metabolize most of these compounds and thus offer a tool for bioremediation purposes. The aim of the present study was to characterize the microbial potential and activity for degradation of aromatic compounds in sediment samples from mangroves using metagenomic and metatranscriptomic approaches. Sediment samples were collected for DNA and RNA extraction from each of the mangrove sites: highly oil-impacted (Oil Mgv), anthropogenically impacted (Ant Mgv) and pristine (Prs Mgv) mangrove. Hydrocarbon concentrations in Oil Mgv sediments were higher than those observed in Ant Mgv and Prs Mgv. Genes and transcripts associated with aromatic compound degradation, particularly the meta and ortho-pathways, were more abundant in Oil Mgv and Ant Mgv suggesting that many of the aromatic compounds are being aerobically degraded by the microbiome in these sites. Functions involved in the degradation of aromatic compounds were also found in pristine site, although in lower abundance. Members of the genera Aromatoleurn, Desulfococcus, Desulfatibacillum, Desulfitobacterium and Vibrio were actively involved in the detoxification of sediments affected by the oil spill. Results obtained from this study provided strong evidence that microbial degradation of aromatic compounds plays an active role in the biological response to mangrove sediment pollution and subsequent ecosystem recovery. (AU)

• Processo FAPESP: 11/50809-5 - Prospecção de metagenoma microbiano de sedimentos de manguezais na busca por novos compostos bioativos
• Beneficiário: Valeria Maia Merzel
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 13/20670-0 - Análise da diversidade funcional e bioprospecção de genes de degradação de hidrocarbonetos e de transformação de metais pesados em sedimentos de manguezal
• Beneficiário: Lucélia Cabral
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 12/06245-2 - Metatranscriptômica e contexto genômico de genes microbianos relacionados aos ciclos biogeoquímicos em manguezais
• Beneficiário: Fernando Dini Andreote
• Modalidade de apoio: Auxílio à Pesquisa - Regular