Insights on the antifungal activity of amphiphilic derivatives of diethylaminoethyl chitosan against Aspergillus flavus

Autor(es)

Dias, Amanda Manchini ^[1] ; dos Santos Cabrera, Marcia Perez ^{[1, 2]} ; Furuyama Lima, Aline Margarete ^[1] ; Taboga, Sebastiao Roberto ^[3] ; Leite Vilamaior, Patricia Simone ^[3] ; Tiera, Marcio Jose ^[1] ; de Oliveira Tiera, Vera Aparecida ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Unesp, Dept Quim & Ciencias Ambientais, Inst Biociencias Letras & Ciencias Exatas Ibilce, Campus Sao Jose do Rio Preto, BR-15054000 Sao Paulo, SP - Brazil

^[2] Univ Estadual Paulista Unesp, Dept Fis, Inst Biociencias Letras & Ciencias Exatas Ibilce, Campus Sao Jose do Rio Preto, BR-15054000 Sao Paulo, SP - Brazil

^[3] Univ Estadual Paulista Unesp, Dept Biol, Inst Biociencias Letras & Ciencias Exatas Ibilce, Campus Sao Jose do Rio Preto, BR-15054000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Carbohydrate Polymers; v. 196, p. 433-444, SEP 15 2018.
• Citações Web of Science: 3

Resumo

In this study, the antifungal activity of chitosan derivatives against A. flavus was studied to understand the contribution of the molecular mass (Mw) and of the hydrophobic and electrostatic forces to the inhibition of fungal growth. The interaction of amphiphilics ranging from 8 to 130 kDa with model membranes of zwitterionic L-alpha-phosphatidylcholine (PC) and anionic L-alpha-phosphatidylcholine/L-alpha-phosphatidyl-DL-glycerol (PC:PG, 80: 20 mol%) were exploited to obtain information on the inhibition mechanism. The results indicated that concurrent interactions control the antifungal activity. The decrease in the Mw weakens the self-association favoring the electrostatic and hydrophobic associations with the cell wall and anionic lipids of the lipid bilayer, indicating an increasing association of the amphiphilics with the fungal membrane. Laser confocal scanning microscopy of rhodamine labeled-derivatives and transmission electronic microscopy techniques showed that the amphiphilics affect the cell wall integrity by inducing the aggregation of hydrophobic constituents of the conidia. (AU)

• Processo FAPESP: 16/15736-0 - "Filmes Biopoliméricos Baseados em Derivados Anfifílicos de Quitosana como Agentes Antimicrobianos: Aplicações e Modo de Ação"
• Beneficiário: Vera Aparecida de Oliveira Tiera
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/08372-7 - Moléculas conjugadas peptídeo/quitosana com potencial farmacologico: síntese, prospecção de atividade em sistemas mimeticos de membrana e avaliações em células
• Beneficiário: Marcia Perez dos Santos Cabrera
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores