Detection of HTLV-1 proviral DNA in BM mononuclear cells and cultured mesenchymal stromal cells isolated from patients with HTLV-1 infection

Autor(es)

Rodrigues, Evandra Strazza ^{[1, 2]} ; Favarin, Maria do Carmo ^[2] ; de Macedo, Mayra Dorigan ^{[1, 2]} ; Otaguiri, Katia Kaori ^{[1, 2]} ; Orellana, Maristela Delgado ^{[3, 2]} ; Takayanagui, Osvaldo Massaiti ^[3] ; Bonini Palma, Patricia Vianna ^[2] ; Slavov, Svetoslav Nanev ^{[3, 2]} ; Covas, Dimas Tadeu ^{[3, 2]} ; Kashima, Simone ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Reg Blood Ctr Ribeirao Preto, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Internal Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY; v. 519, p. 145-155, JUN 2018.
• Citações Web of Science: 0

Resumo

The bone marrow (BM) biology during HTLV-1 infection is obscure. In this study, we investigated BM mononuclear cells and mesenchymal stromal cells (MSC) from HTLV-1 asymptomatic and symptomatic individuals. An infiltration of CD4(+)T-cell lymphocytes in the BM of HTLV-1-infected individuals was observed when compared to healthy controls. The provirus detection in the BM CD4(+)T cells confirmed the presence of integrated HTLV DNA. In regard to MSC, we observed that the number of fibroblast progenitor cells was lower in HTLV-1 infected individuals than in healthy controls. Isolated HTLV-1 infected BM-MSC demonstrated surface expression markers and in vitro differentiation potential similar to uninfected individuals. The presence of HTLV-1 proviral DNA in the BM-MSC of HTLV-1-infected patients was demonstrated but no p19 antigen was detected in supernatant from cultured MSC. We suppose that HTLV-1 infects human MSC probably by cell-to-cell contact from the infected CD4(+)T-lymphocytes infiltrated into the bone marrow. (AU)

• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs