Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Detection of HTLV-1 proviral DNA in BM mononuclear cells and cultured mesenchymal stromal cells isolated from patients with HTLV-1 infection

Full text
Author(s):
Rodrigues, Evandra Strazza [1, 2] ; Favarin, Maria do Carmo [2] ; de Macedo, Mayra Dorigan [1, 2] ; Otaguiri, Katia Kaori [1, 2] ; Orellana, Maristela Delgado [3, 2] ; Takayanagui, Osvaldo Massaiti [3] ; Bonini Palma, Patricia Vianna [2] ; Slavov, Svetoslav Nanev [3, 2] ; Covas, Dimas Tadeu [3, 2] ; Kashima, Simone [1, 2]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, Sao Paulo - Brazil
[2] Univ Sao Paulo, Reg Blood Ctr Ribeirao Preto, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Internal Med, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: VIROLOGY; v. 519, p. 145-155, JUN 2018.
Web of Science Citations: 0
Abstract

The bone marrow (BM) biology during HTLV-1 infection is obscure. In this study, we investigated BM mononuclear cells and mesenchymal stromal cells (MSC) from HTLV-1 asymptomatic and symptomatic individuals. An infiltration of CD4(+)T-cell lymphocytes in the BM of HTLV-1-infected individuals was observed when compared to healthy controls. The provirus detection in the BM CD4(+)T cells confirmed the presence of integrated HTLV DNA. In regard to MSC, we observed that the number of fibroblast progenitor cells was lower in HTLV-1 infected individuals than in healthy controls. Isolated HTLV-1 infected BM-MSC demonstrated surface expression markers and in vitro differentiation potential similar to uninfected individuals. The presence of HTLV-1 proviral DNA in the BM-MSC of HTLV-1-infected patients was demonstrated but no p19 antigen was detected in supernatant from cultured MSC. We suppose that HTLV-1 infects human MSC probably by cell-to-cell contact from the infected CD4(+)T-lymphocytes infiltrated into the bone marrow. (AU)

FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC