| Texto completo | |
| Autor(es): |
Ikegami, Amanda
[1]
;
Teixeira, Luiz Felipe S.
[1]
;
Braga, Marina S.
[2]
;
Dias, Matheus Henrique Dos S.
[3]
;
Lopes, Eduardo C.
[3]
;
Bellini, Maria Helena
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] IPEN CNEN SP, Dept Biotechnol, 2242 Av Lineu Prestes, Cidade Univ, BR-05508000 Butanta, SP - Brazil
[2] Univ Sao Paulo, Dept Pharm, Fac Pharmaceut Sci, Sao Paulo - Brazil
[3] Inst Butantan, LETA, Ctr Toxins Immune Response & Cell Signaling CeTIC, Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ONCOLOGY RESEARCH; v. 26, n. 5, p. 743-751, 2018. |
| Citações Web of Science: | 0 |
| Resumo | |
Renal cell carcinoma (RCC) accounts for approximately 2%-3% of human malignancies and is the most aggressive among urologic tumors. Biological heterogeneity, drug resistance, and chemotherapy side effects are the biggest obstacles to the effective treatment of RCC. The NF-kappa B transcription factor is one of several molecules identified to be responsible for the aggressive phenotype of this tumor. In the past decade, several studies have demonstrated the activation of NF-kappa B in RCC, and many have implicated NF-kappa B1 (p50) as an important molecule in tumor progression and metastasis. In the present study, a lentivirus was used to deliver shRNA targeting NF-kappa B1 into mouse RCC (Renca) cells. It was determined that the knockdown of the NF-kappa B1 gene led to a reduction in cell proliferation and late apoptosis/necrosis in vitro. Flow cytometry analysis demonstrated G(2)/M arrest in the cells. In addition, immunoblotting analysis revealed a significant increase in cyclin B1 and Bax. In vivo experiments showed that Renca-shRNA-NF-kappa B1 cells have significantly diminished tumori genicity. Moreover, immunohistochemical analysis revealed an increase in necrotic areas of Renca-shRNA-NF-kappa B1 tumors. Thus, this study indicates that downregulation of NF-kappa B1 can suppress RCC tumorigenesis by inducing late apoptosis/necrosis. Therefore, NF-kappa B1 may be a potential therapeutic target for RCC. (AU) | |
| Processo FAPESP: | 14/19265-7 - Avaliação funcional do gene NF-Kb1 na tumorigênese de linhagens celulares de carcinoma renal |
| Beneficiário: | Maria Helena Bellini Marumo |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |