Central fractalkine stimulates central prostaglandin E-2 production and induces systemic inflammatory responses

Autor(es)

Mota, Clarissa M. D. ^[1] ; Antunes-Rodrigues, Jose ^[1] ; Branco, Luiz G. S. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Physiol, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Morphol Physiol & Basic Pathol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brain Research Bulletin; v. 140, p. 311-317, JUN 2018.
• Citações Web of Science: 0

Resumo

Fractalkine (FKN; CX3CL1) belongs to gamma-chemokine family and binds to CX3CR1 receptors. Currently, the mechanisms involving FKN-induced inflammatory mediators are research targets in an attempt to study immune diseases mechanisms. Besides, FKN seems to modulate inflammation in the nervous system by inducing the secretion of pro-inflammatory mediators such as prostaglandin E-2 (PGE(2)). PGE(2) is a classic and important mediator of fever that activates warm-responsive neurons in the anteroventral preoptic region of the hypothalamus (AVPO). Here, we tested the hypothesis that central FKN modulates febrigenic signaling both centrally and peripherally. We performed intracerebroventricular (icv) microinjections of saline (1 mu L) or FKN (doses of 5, 50, 500 pg/mu L) in rats and measured body temperature (Tb) besides assessing tail skin temperature (Tsk) as a thermoeffector indicator used to calculate the heat loss index (HLI). We also measured the time course changes in AVPO PGE(2), besides plasma corticosterone (CORT) and interleukin-6 (IL-6) levels. FKN induced a long lasting febrile response in which the highest dose (500 pg/mu L) induced a marked rise on Tb that was accompanied by a reduced Tsk and HLI, consequently. FKN increased AVPO PGE(2) production in a time-dependent manner besides increasing plasma CORT and IL-6 levels. Our data consistently indicate that FKN increases AVPO PGE(2) production and Tb, accompanied by raised plasma IL-6 levels and activation of the hypothalamus-pituitary-adrenal axis. (AU)

• Processo FAPESP: 17/01633-8 - Papel da serotonina na área pré-óptica na modulação da temperatura corporal e da nocicepção durante a inflamação sistêmica
• Beneficiário: Clarissa Maria Dias Mota
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/17681-9 - Alterações fisiopatológicas durante a inflamação sistêmica
• Beneficiário: Luiz Guilherme de Siqueira Branco
• Modalidade de apoio: Auxílio à Pesquisa - Temático