Induction of Type 2 Iodothyronine Deiodinase After Status Epilepticus Modifies Hippocampal Gene Expression in Male Mice

Autor(es):Mostrar menos -

Nascimento, Bruna P. P. ^{[1, 2]} ; Bocco, Barbara M. L. C. ^[3] ; Fernandes, Gustavo W. ^[3] ; Fonseca, Tatiana L. ^[3] ; McAninch, Elizabeth A. ^[3] ; Cardoso, Carolina V. ^[4] ; Bondan, Eduardo F. ^[4] ; Nassif, Renata J. ^[5] ; Cysneiros, Roberta M. ^[1] ; Bianco, Antonio C. ^[6] ; Ribeiro, Miriam O. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Prebiteriana Mackenzie, Dev Disorders Program, Ctr Biol Sci & Hlth, BR-01302900 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Med, Grad Program Translat Med, BR-04039002 Sao Paulo, SP - Brazil

^[3] Rush Univ, Div Endocrinol & Metab, Dept Internal Med, Med Ctr, Chicago, IL 60612 - USA

^[4] Univ Paulista, Dept Environm & Expt Pathol, BR-04026002 Sao Paulo, SP - Brazil

^[5] Univ Fed Sao Paulo, Dept Neurol & Neurosurg, Neurosci Sect, BR-04039032 Sao Paulo, SP - Brazil

^[6] Univ Chicago, Div Endocrinol, Dept Med, Chicago, IL 60612 - USA

• Tipo de documento: Artigo Científico
• Fonte: Endocrinology; v. 159, n. 8, p. 3090-3104, AUG 2018.
• Citações Web of Science: 2

Resumo

Status epilepticus (SE) is an abnormally prolonged seizure that results from either a failure of mechanisms that terminate seizures or from initiating mechanisms that inherently lead to prolonged seizures. Here we report that mice experiencing a 3 hours of SE caused by pilocarpine exhibit a rapid increase in expression of type 2 iodothyronine deiodinase gene (Dio2) and a decrease in the expression of type 3 iodothyronine deiodinase gene in hippocampus, amygdala and prefrontal cortex. Type 3 iodothyronine deiodinase in hippocampal sections was seen concentrated in the neuronal nuclei, typical of ischemic injury of the brain. An unbiased analysis of the hippocampal transcriptome of mice undergoing 3 hours of SE revealed a number of genes, including those involved with response to oxidative stress, cellular homeostasis, cell signaling, and mitochondria! structure. In contrast, in mice with targeted disruption of Dio2 in astrocytes (Astro D2KO mouse), the highly induced genes in the hippocampus were related to inflammation, apoptosis, and cell death. We propose that Dio2 induction caused by SE accelerates production of T3 in different areas of the central nervous system and modifies the hippocampal gene expression profile, affecting the balance between adaptive and maladaptive mechanisms. (AU)

• Processo FAPESP: 13/09133-3 - Papel da iodotironina desiodase tipo 3 no modelo de epilepsia do lobo temporal induzida por pilocarpina em camundongos
• Beneficiário: Bruna Pascarelli Pedrico Do Nascimento
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/13235-1 - Sinalização do hormônio tireoidiano no hipocampo de ratos obesos.
• Beneficiário: Miriam Oliveira Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular